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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Label-Free Quantitative Proteomic Analysis of Puccinia psidii Uredospores Reveals Differences of Fungal Populations Infecting Eucalyptus and Guava

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Author(s):
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Quecine, Maria Carolina [1] ; Leite, Thiago Falda [1] ; Bini, Andressa Peres [1] ; Regiani, Thais [1] ; Franceschini, Livia Maria [1] ; Frasson Budzinski, Ilara Gabriela [1] ; Marques, Felipe Garbelini [1] ; Veneziano Labate, Monica Teresa [1] ; Guidetti-Gonzalez, Simone [1] ; Moon, David Henry [1] ; Labate, Carlos Alberto [1]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, Piracicaba, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: PLoS One; v. 11, n. 1 JAN 5 2016.
Web of Science Citations: 4
Abstract

Puccinia psidii sensu lato (s.l.) is the causal agent of eucalyptus and guava rust, but it also attacks a wide range of plant species from the myrtle family, resulting in a significant genetic and physiological variability among populations accessed from different hosts. The uredo-spores are crucial to P. psidii dissemination in the field. Although they are important for the fungal pathogenesis, their molecular characterization has been poorly studied. In this work, we report the first in-depth proteomic analysis of P. psidii s.l. uredospores from two contrasting populations: guava fruits (PpGuava) and eucalyptus leaves (PpEucalyptus). NanoUPLC-MSE was used to generate peptide spectra that were matched to the UniProt Puccinia genera sequences (UniProt database) resulting in the first proteomic analysis of the phytopathogenic fungus P. psidii. Three hundred and fourty proteins were detected and quantified using Label free proteomics. A significant number of unique proteins were found for each sample, others were significantly more or less abundant, according to the fungal populations. In PpGuava population, many proteins correlated with fungal virulence, such as malate dehydrogenase, proteossomes subunits, enolases and others were increased. On the other hand, PpEucalyptus proteins involved in biogenesis, protein folding and translocation were increased, supporting the physiological variability of the fungal populations according to their protein reservoirs and specific host interaction strategies. (AU)

FAPESP's process: 08/50361-1 - Functional genomics applied to the discovery of eucalyptus rust resistance genes
Grantee:Carlos Alberto Labate
Support type: Research Grants - Research Partnership for Technological Innovation - PITE
FAPESP's process: 11/17455-5 - Analysis of total metabolites and proteins in leaves of Eucalyptus grandis during infection by Puccinia psidii
Grantee:Felipe Garbelini Marques
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 12/22227-4 - Global analysis of the molecular mechanisms involved in drought tolerance and sucrose accumulation in two varieties of cane sugar
Grantee:Ilara Gabriela Frasson Budzinski
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/07596-6 - Molecular study of the pathogenic fungus Puccinia psidii Winter, the causal agent of rust, in vitro and during their interaction with Eucalyptus spp
Grantee:Andressa Peres Bini
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 10/15417-6 - Proteome and fosfoproteome of sugarcane under water stress
Grantee:Simone Guidetti Gonzalez
Support type: Scholarships in Brazil - Post-Doctorate