Global analysis of the molecular mechanisms involved in drought tolerance and sucrose accumulation in two varieties of cane sugar

• Grant number: 12/22227-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): July 01, 2013
• Effective date (End): June 30, 2016
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Carlos Alberto Labate
• Grantee: Ilara Gabriela Frasson Budzinski
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant: 08/56100-5 - Metabolomics of sugarcane and discovery of new hydrolytic enzymes for biofuel production, AP.BIOEN.TEM

Abstract

The Brazilian Etanol and Sugar Production areas are expanding, including areas of the Midwest and Northeast where water availability is scarce or irregular. The establishment of the crop in the field is critical to the agriculture success, since it defines the potential production thereof directly reflecting in the national industry. Thus, it is of fundamental importance to understand the molecular and biochemical mechanisms that regulate drought tolerance and accumulation of sucrose in sugarcane. In this context, this project proposes to study the global profile of transcripts, proteins and metabolites in leaves and stems of two varieties of sugarcane contrasting in drought tolerance. To this end we propose transcripts analyzes by q PCR, proteomic analysis by bidimensional gels and subsequent protein identification by MALDI-TOF-MS. We also propose the analysis of primary and secondary metabolites by gas and liquid chromatography associated to mass spectrometry. The data generated will permit the identification of genes, proteins and potential pathways for more detailed studies. The data can also result in the identification of molecular markers, which may be used in experiments of assisted selection. Moreover, the information obtained can also be extrapolated to understand the mechanisms of drought tolerance and sucrose accumulation in other plant species. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BUDZINSKI, ILARA GABRIELA FRASSON; DE MORAES, FABRICIO EDGAR; CATALDI, THAIS REGIANI; FRANCESCHINI, LIVIA MARIA; LABATE, CARLOS ALBERTO. Network Analyses and Data Integration of Proteomics and Metabolomics From Leaves of Two Contrasting Varieties of Sugarcane in Response to Drought. FRONTIERS IN PLANT SCIENCE, v. 10, NOV 28 2019. (12/22227-4, 12/12521-2)

BOARETTO, LUIS FELIPE; VENEZIANO LABATE, MONICA TERESA; FRANCESCHINI, LIVIA MARIA; CATALDI, THAIS REGIANI; BUDZINSKI, ILARA GABRIELA F.; DE MORAES, FABRICIO EDGAR; LABATE, CARLOS ALBERTO. Proteomics Reveals an Increase in the Abundance of Glycolytic and Ethanolic Fermentation Enzymes in Developing Sugarcane Culms During Sucrose Accumulation. FRONTIERS IN PLANT SCIENCE, v. 12, SEP 30 2021. (12/22227-4, 08/56100-5, 12/12521-2)

QUECINE, MARIA CAROLINA; LEITE, THIAGO FALDA; BINI, ANDRESSA PERES; REGIANI, THAIS; FRANCESCHINI, LIVIA MARIA; FRASSON BUDZINSKI, ILARA GABRIELA; MARQUES, FELIPE GARBELINI; VENEZIANO LABATE, MONICA TERESA; GUIDETTI-GONZALEZ, SIMONE; MOON, DAVID HENRY; et al. Label-Free Quantitative Proteomic Analysis of Puccinia psidii Uredospores Reveals Differences of Fungal Populations Infecting Eucalyptus and Guava. PLoS One, v. 11, n. 1, JAN 5 2016. (08/50361-1, 10/15417-6, 13/07596-6, 12/22227-4, 11/17455-5)