Metabolomics of sugarcane and discovery of new hydrolytic enzymes for biofuel production

• Grant number: 08/56100-5
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• Duration: November 01, 2010 - October 31, 2014
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Convênio/Acordo: CNPq - Pronex
• Principal Investigator: Carlos Alberto Labate
• Grantee: Carlos Alberto Labate
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated scholarship(s): 12/22227-4 - Global analysis of the molecular mechanisms involved in drought tolerance and sucrose accumulation in two varieties of cane sugar, BP.PD; 12/12521-2 - Bioinformatics applied to systems biology for regulatory factors identification of the sucrose accumulation in sugarcane stalk, BP.DR; 12/04182-3 - Metabolomics of the sugar accumulation in sugar cane (Saccharum L.), BP.PD; 10/15417-6 - Proteome and fosfoproteome of sugarcane under water stress, BP.PD

Abstract

Sugarcane bagasse and Eucalyptus are the main renewable feedstock available in Brazil for biofuel production. The project has two main lines of research: in the first part of we will characterize the metabolome of different tissues and organs of sugarcane varieties and, grown under different experimental conditions of biotic and abiotic stresses (drought, high and low temperature, pathogens, insects, high CO2, etc). The aim is to develop a data bank of metabolites and metabolic networks linking the transcriptomics information, already available, to identify new gene functions and phenotypes. All the information from sugarcane will be integrated into the bioinformatics server of SUCEST allowing the user to crosslink the data of experiments from transcriptomics, proteomics and metabolomics, molecular markers (such as QTLS and E-QTLS) or any other service available in the SUCEST server. The second part of the project will evaluate the potential to isolate new cellulases, hemicellulases and lignin degrading enzymes from microorganisms and insects occurring in areas of commercial plantations of eucalypts in Brazil, particularly in areas of reform. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CALDERAN-RODRIGUES, MARIA JULIANA; JAMET, ELISABETH; DOUCHE, THIBAUT; RODRIGUES BONASSI, MARIA BEATRIZ; CATALDI, THAIS REGIANI; FONSECA, JULIANA GUIMARAES; SAN CLEMENTE, HELENE; PONT-LEZICA, RAFAEL; LABATE, CARLOS ALBERTO. Cell wall proteome of sugarcane stems: comparison of a destructive and a non-destructive extraction method showed differences in glycoside hydrolases and peroxidases. BMC PLANT BIOLOGY, v. 16, JAN 11 2016. (08/56100-5)

CALDERAN-RODRIGUES, MARIA JULIANA; JAMET, ELISABETH; CALDERAN RODRIGUES BONASSI, MARIA BEATRIZ; GUIDETTI-GONZALEZ, SIMONE; BEGOSSI, AMANDA CARMANHANIS; SETEM, LAIS VAZ; FRANCESCHINI, LIVIA MARIA; FONSECA, JULIANA GUIMARAES; LABATE, CARLOS ALBERTO. Cell wall proteomics of sugarcane cell suspension cultures. PROTEOMICS, v. 14, n. 6, p. 738-749, MAR 2014. (08/56100-5, 10/15417-6)

FONSECA, JULIANA G.; CALDERAN-RODRIGUES, MARIA J.; DE MORAES, FABRICIO E.; CATALDI, THAIS R.; JAMET, ELISABETH; LABATE, CARLOS A.. Cell Wall Proteome of Sugarcane Young and Mature Leaves and Stems. PROTEOMICS, v. 18, n. 2, JAN 2018. (08/56100-5, 13/08091-5)

BOARETTO, LUIS FELIPE; VENEZIANO LABATE, MONICA TERESA; FRANCESCHINI, LIVIA MARIA; CATALDI, THAIS REGIANI; BUDZINSKI, ILARA GABRIELA F.; DE MORAES, FABRICIO EDGAR; LABATE, CARLOS ALBERTO. Proteomics Reveals an Increase in the Abundance of Glycolytic and Ethanolic Fermentation Enzymes in Developing Sugarcane Culms During Sucrose Accumulation. FRONTIERS IN PLANT SCIENCE, v. 12, SEP 30 2021. (12/22227-4, 08/56100-5, 12/12521-2)

CALDERAN-RODRIGUES, MARIA JULIANA; FONSECA, JULIANA GUIMARAES; DE MORAES, FABRICIO EDGAR; SETEM, LAIS VAZ; BEGOSSI, AMANDA CARMANHANIS; LABATE, CARLOS ALBERTO. Plant Cell Wall Proteomics: A Focus on Monocot Species, Brachypodium distachyon, Saccharum spp. and Oryza sativa. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 8, APR 2 2019. (08/56100-5)