Production of biosurfactants from vine-trimming shoots using the halotolerant strain Bacillus tequilensis ZSB10

Autor(es)

Cortes-Camargo, Stefani ^[1] ; Perez-Rodriguez, Noelia ^{[2, 3]} ; de Souza Oliveira, Ricardo Pinheiro ^[4] ; Barragan Huerta, Blanca E. ^[1] ; Manuel Dominguez, Jose ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] IPN, Unidad Profes Adolfo Loipez Mateos, Escuela Nacl Ciencias Biol, Dept Ingn Sistemas Ambientales, Mexico City 07738, DF - Mexico

^[2] Univ Vigo, Fac Sci, Dept Chem Engn, Orense 32004 - Spain

^[3] Univ Vigo, CITI, Lab Agrofood Biotechnol, San Cibrao Das Vinas, Ourense - Spain

^[4] Univ Sao Paulo, Fac Pharmaceut Sci, Biochem & Pharmaceut Technol Dept, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INDUSTRIAL CROPS AND PRODUCTS; v. 79, p. 258-266, JAN 2016.
• Citações Web of Science: 8

Resumo

The strain Bacillus tequilensis ZSB10, isolated from Mexican brines, was able to grow and produce extracellular and cell-bound biosurfactants using nine culture broths formulated from hydrolyzates obtained from the cellulosic and hemicellulosic fractions of vine-trimming wastes. The results confirm its halotolerance since it managed to grow both in the presence and absence of salts. It also was able to consume sugars such as glucose and xylose. The process was then scaled up into a 2-L bioreactor using the mixture of hemicellulosic (50%) and cellulosic hydrolyzate (50%) supplemented with mineral salt medium as culture medium at different biomass concentrations. Crude extracellular biosurfactant yielded 1.52 g/L and lowered the surface tension to 38.6 mN/m with a critical micelle concentration of 177.14 mg/L. Furthermore, it was able to emulsify with kerosene after 24h (E-24 = 47%). Crude cell-bound biosurfactant only yielded 0.0783 g/L and showed lower emulsifying characteristics than extracellular biosurfactant (E-24 = 41% with kerosene). (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/19997-5 - Processo de internacionalização em pesquisa biotecnológica entre as Universidades de São Paulo e Vigo
• Beneficiário: Ricardo Pinheiro de Souza Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional