Polymorphism of Metallothionein 2A Modifies Lead Body Burden in Workers Chronically Exposed to the Metal

Autor(es)

Marques Fernandes, Kelly Christine ^[1] ; Martins, Jr., Airton Cunha ^[1] ; Soares de Oliveira, Andreia Avila ^[1] ; Greggi Antunes, Lusania Maria ^[1] ; de Syllos Colus, Ilce Mara ^[2] ; Barbosa, Jr., Fernando ^[1] ; Mazzaron Barcelos, Gustavo Rafael ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Clin Anal Toxicol & Food Sci, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Estadual Londrina, Dept Gen Biol, Ctr Biol Sci, Londrina - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PUBLIC HEALTH GENOMICS; v. 19, n. 1, p. 47-52, 2016.
• Citações Web of Science: 9

Resumo

Lead (Pb) is a metal that accumulates in the human body, inducing several adverse health effects. One of the proteins responsible for the distribution of metal in the body is metallothionein (MT), which is expressed by different genes, and it is supposed that genetic variation in the genes that encode MTs may affect the Pb body burden. The present study aimed to evaluate the genetic effects of the polymorphism of MT2A (single nucleotide polymorphism rs10636; C -> G) on blood Pb levels (BLL) of workers from car battery factories who are chronically exposed to the metal. In total, 221 men participated in the study; genomic DNA from whole blood was extracted, and genotyping of MT2A was performed by TaqMan assays; BLL were quantified by inductively coupled plasma mass spectrometry (ICP-MS). BLL were 25 +/- 14 mu g/dI (range 1.9-68); BLL were positively correlated with duration of work and smoking status. Individuals who carried at least one C allele had higher BLL than those with the GG genotype (beta = 0.45; p = 0.025, multivariable linear regression analyses). Taken together, our data support the hypothesis that polynnorphisms in genes related to the transport of Pb, such as MTs, may modulate the concentrations of the metal in the body and, consequently, adverse health effects induced by Pb exposure. (C) 2015 S. Karger AG, Basel (AU)

• Processo FAPESP: 13/06033-8 - Integração de ferramentas ômicas para o estudo de modelos multivariados nas interações gene-ambiente em população expostas a metais tóxicos
• Beneficiário: Gustavo Rafael Mazzaron Barcelos
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 13/17288-7 - Avaliação de efeitos genéticos dos polimorfismos das metalotioneínas MT1a e MT2a sobre as concentrações de chumbo em trabalhadores expostos ao metal
• Beneficiário: Kelly Christine Marques Fernandes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica