| Texto completo | |
| Autor(es): |
Lario, Luciana Daniela
[1]
;
Pellegrini Malpiedi, Luciana
[1, 2]
;
Brandao Pereira, Jorge Fernando
[3]
;
Sette, Lara Duraes
[4]
;
Pessoa-Junior, Adalberto
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Biochem & Pharmaceut Technol, Sao Paulo - Brazil
[2] Univ Nacl Rosario, Fac Ciencias Bioquim & Farmaceut, Dept Quim Fis, RA-2000 Rosario, Santa Fe - Argentina
[3] Univ Estadual Paulista, UNESP, Sch Pharmaceut Sci, Dept Bioproc & Biotechnol, Araraquara, SP - Brazil
[4] Univ Sao Paulo UNESP, Inst Biosci, Dept Biochem & Microbiol, Sao Paulo - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | SEPARATION SCIENCE AND TECHNOLOGY; v. 51, n. 1, p. 57-67, JAN 2 2016. |
| Citações Web of Science: | 6 |
| Resumo | |
This work aimed to optimize the extraction of an extracellular protease produced by the cold-adapted yeast Rhodotorula mucilaginosa L7 using aqueous two-phase systems (ATPS) comprising polyethylene glycol (PEG) and sodium citrate or sodium tartrate. First, the biocompatibility of the phase forming agents was assessed. The results obtained with PEG-2000, PEG-4000, and PEG-6000 demonstrated that even at large PEG concentrations (32 wt%) the protease maintains its activity after 3 h of reaction, whereas an increase in salt concentration provokes a gradual decrease in protease stability. Subsequently, the partitioning of the protease in both types of ATPS was assessed, evaluating the effect of temperature, molecular weight, and concentration of PEG on protease purification, using two 2(3)-full factorial designs. The best partitioning conditions were obtained in PEG-6000/sodium tartrate-based ATPS, at 30oC (with a yield of 81.09 +/- 0.66% and a purification factor of 2.51 +/- 0.03). Thus, considering the biodegradable characteristics of the system, the PEG/sodium tartrate ATPS is a viable and economic low-resolution step in protease purification, with a strong potential for future industrial application. (AU) | |
| Processo FAPESP: | 13/19486-0 - Biotecnologia marinha e Antártica: enzimas microbianas e suas aplicações |
| Beneficiário: | Lara Durães Sette |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/20521-0 - Extração de fragmentos de anticorpos monoclonais scFv por sistemas micelares de duas fases aquosas |
| Beneficiário: | Luciana Pellegrini Malpiedi |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 12/23726-4 - "purificação de enzimas proteolíticas secretadas pela levedura psicrótrofa Rhodoturola mucilaginosa por sistemas de duas fases aquosas (PEG/NaPA)e processos cromatográficos" |
| Beneficiário: | Luciana Daniela Lario |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |