| Texto completo | |
| Autor(es): |
Castro, Lilian dos Santos
[1]
;
de Paula, Renato G.
[1]
;
Antonieto, Amanda C. C.
[1]
;
Persinoti, Gabriela F.
[2]
;
Silva-Rocha, Rafael
[3]
;
Silva, Roberto N.
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Mol Biotechnol Lab, BR-14049 Ribeirao Preto - Brazil
[2] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Ciencia & Tecnol Bioetanol, Campinas, SP - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell & Mol Biol, Syst & Synthet Biol Lab, BR-14049 Ribeirao Preto - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FRONTIERS IN MICROBIOLOGY; v. 7, FEB 16 2016. |
| Citações Web of Science: | 19 |
| Resumo | |
We defined the role of the transcriptional factor XYR1 in the filamentous fungus Trichoderma reesei during cellulosic material degradation. In this regard, we performed a global transcriptome analysis using RNA-Seq of the Axyrl mutant strain of T reesei compared with the parental strain QM9414 grown in the presence of cellulose, sophorose, and glucose as sole carbon sources. We found that 5885 genes were expressed differentially under the three tested carbon sources. Of these, 322 genes were upregulated in the presence of cellulose, while 367 and 188 were upregulated in sophorose and glucose, respectively. With respect to genes under the direct regulation of XYR1, 30 and 33 are exclusive to cellulose and sophorose, respectively. The most modulated genes in the Axyrl belong to Carbohydrate-Active Enzymes (CAZymes), transcription factors, and transporters families. Moreover, we highlight the downregulation of transporters belonging to the MFS and ABC transporter families. Of these, MFS members were mostly downregulated in the presence of cellulose. In sophorose and glucose, the expression of these transporters was mainly upregulated. Our results revealed that MFS and ABC transporters could be new players in cellulose degradation and their role was shown to be carbon source-dependent. Our findings contribute to a better understanding of the regulatory mechanisms of XYR1 to control cellulase gene expression in T reesei in the presence of cellulosic material, thereby potentially enhancing its application in several biotechnology fields. (AU) | |
| Processo FAPESP: | 10/15683-8 - Estudos de sinalização celular e mecanismos de indução da formação de celulases pelo fungo Trichoderma reesei (Hypocrea jecorina) |
| Beneficiário: | Roberto do Nascimento Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores |
| Processo FAPESP: | 14/23653-2 - Caracterização de novos transportadores de açúcar envolvidos na regulação da degradação da biomassa lignocelulósica em Trichoderma reesei |
| Beneficiário: | Roberto do Nascimento Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |