Understanding the Role of the Master Regulator XYR1 in Trichoderma reesei by Global Transcriptional Analysis

Author(s)

Castro, Lilian dos Santos ^[1] ; de Paula, Renato G. ^[1] ; Antonieto, Amanda C. C. ^[1] ; Persinoti, Gabriela F. ^[2] ; Silva-Rocha, Rafael ^[3] ; Silva, Roberto N. ^[1]

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Mol Biotechnol Lab, BR-14049 Ribeirao Preto - Brazil

^[2] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Ciencia & Tecnol Bioetanol, Campinas, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell & Mol Biol, Syst & Synthet Biol Lab, BR-14049 Ribeirao Preto - Brazil

• Document type: Journal article
• Source: FRONTIERS IN MICROBIOLOGY; v. 7, FEB 16 2016.
• Web of Science Citations: 19

Abstract

We defined the role of the transcriptional factor XYR1 in the filamentous fungus Trichoderma reesei during cellulosic material degradation. In this regard, we performed a global transcriptome analysis using RNA-Seq of the Axyrl mutant strain of T reesei compared with the parental strain QM9414 grown in the presence of cellulose, sophorose, and glucose as sole carbon sources. We found that 5885 genes were expressed differentially under the three tested carbon sources. Of these, 322 genes were upregulated in the presence of cellulose, while 367 and 188 were upregulated in sophorose and glucose, respectively. With respect to genes under the direct regulation of XYR1, 30 and 33 are exclusive to cellulose and sophorose, respectively. The most modulated genes in the Axyrl belong to Carbohydrate-Active Enzymes (CAZymes), transcription factors, and transporters families. Moreover, we highlight the downregulation of transporters belonging to the MFS and ABC transporter families. Of these, MFS members were mostly downregulated in the presence of cellulose. In sophorose and glucose, the expression of these transporters was mainly upregulated. Our results revealed that MFS and ABC transporters could be new players in cellulose degradation and their role was shown to be carbon source-dependent. Our findings contribute to a better understanding of the regulatory mechanisms of XYR1 to control cellulase gene expression in T reesei in the presence of cellulosic material, thereby potentially enhancing its application in several biotechnology fields. (AU)

• FAPESP's process: 10/15683-8 - Studies of cellular signaling and induction mechanisms of cellulases formation by the fungus Trichoderma reesei (Hypocrea jecorina)
• Grantee: Roberto do Nascimento Silva
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
• FAPESP's process: 14/23653-2 - Characterization of new sugar transporters involved in the regulation of lignocellulosic biomass degradation of in Trichoderma reesei
• Grantee: Roberto do Nascimento Silva
• Support Opportunities: Regular Research Grants