Vaginal Inflammation: Association between Leukocyte Concentration and Levels of Immune Mediators

Autor(es)

Beghini, Joziani ^[1] ; Giraldo, Paulo C. ^[1] ; Eleuterio, Jr., Jose ^[2] ; do Amaral, Rose Luce ^[1] ; Polpeta, Nadia C. ^[1] ; Goncalves, Ana Katherine ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Gynecol & Obstet, Campinas, SP - Brazil

^[2] Univ Fed Ceara, Dept Motherhood & Child, Fortaleza, Ceara - Brazil

^[3] Univ Fed Rio Grande do Norte, Dept Gynecol & Obstet, BR-59072970 Natal, RN - Brazil

• Tipo de documento: Artigo Científico
• Fonte: American Journal of Reproductive Immunology; v. 75, n. 2, p. 126-133, FEB 2016.
• Citações Web of Science: 1

Resumo

Problem A wide variety of mediators are involved in inflammatory processes. However, the identity of those participating in vaginal immune responses has not been established. We correlated extracellular matrix metalloproteinase inducer (EMMPRIN), matrix metalloproteinase-8 (MMP-8), hyaluronan (HA), hyaluronidase-1 (Hyal-1), human beta-defensin-2 (hBD2), and neutrophil gelatinase-associated lipocalin (NGAL) concentrations with the extent of leukocyte infiltration into the vagina and suggest their participation in vaginal inflammation. Methods of study Vaginal fluid was obtained from 233 women seen at the outpatient clinic in the Department of Obstetrics and Gynecology at Campinas University, Brazil. The magnitude of vaginal inflammation was determined by the leukocyte count on vaginal smears and categorized as no inflammation (0 leukocytes/field), moderate inflammation (1-4 leukocytes/field), and intense inflammation (>4 leukocytes/field). Concentrations of EMMPRIN, MMP-8, HA, Hyal-1, hBD2, and NGAL were determined in vaginal fluid by ELISA. Results EMMPRIN, MMP-8, HA, hBD2, and NGAL concentration increased with elevated leukocyte numbers (P < 0.05), while Hyal-1 did not. EMMPRIN concentrations were correlated with HA and MMP-8 levels. Conclusion EMMPRIN, MMP-8, HA, b-defensin, and NGAL are elevated in women with vaginal inflammation. (AU)

• Processo FAPESP: 11/19960-9 - Resposta humoral cérvico-vaginal (IgA secretora) em mulheres que receberam a vacina quadrivalente contra o HPV
• Beneficiário: Paulo César Giraldo
• Modalidade de apoio: Auxílio à Pesquisa - Regular