Layer-by-layer films containing peptides of the Cry1Ab16 toxin from Bacillus thuringiensis for potential biotechnological applications

Autor(es)

Placido, Alexandra ^[1] ; de Oliveira Farias, Emanuel Airton ^[2] ; Marani, Mariela M. ^[3] ; Vasconcelos, Andreanne G. ^[2] ; Mafud, Ana C. ^[4] ; Mascarenhas, Yvonne P. ^[4] ; Eiras, Carla ^{[2, 5]} ; Leite, Jose R. S. A. ^[2] ; Delerue-Matos, Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Inst Politecn Porto, Inst Super Engn Porto, REQUIMTE LAQV, Rua Dr Antonio Bernardino Almeida 431, P-4200072 Oporto - Portugal

^[2] Univ Fed Piaui, CMRV, BIOTEC, Nucl Pesquisa Biodiversidade & Biotechnol, Campus Ministro Reis Velloso, BR-64202020 Parnaiba, Piaui - Brazil

^[3] Consejo Nacl Invest Cient & Tecn, Ctr Nacl Patagon, IPEEC CENPAT CONICET, RA-9120 Puerto Madryn, Chubut - Argentina

^[4] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

^[5] Univ Fed Piaui, Ctr Tecnol, LIMAV, Lab Mat Avancados, Engn Mat, BR-64049550 Teresina, Piaui - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 61, p. 832-841, APR 1 2016.
• Citações Web of Science: 8

Resumo

CrylAbl6 is a toxin of crystalline insecticidal proteins that has been widely used in genetically modified organisms (GMOs) to gain resistance to pests. For the first time, in this study, peptides derived from the immunogenic CrylAb16 toxin (from Bacillus thuringiensis) were immobilized as layer -by -layer (LbL) films. Given the concern about food and environmental safety, a peptide with immunogenic potential, PcL342-354C, was selected for characterization of the electrochemical, optical, and morphological properties. The results obtained by cyclic voltammetry (CV) showed that the peptide have an irreversible oxidation process in electrolyte of 0.1 mol `` L-1 potassium phosphate buffer (PBS) at pH 7.2. It was also observed that the electrochemical response of the peptide is governed mainly by charge transfer. In an attempt to maximize the electrochemical signal of peptide, it was intercalated with natural (agar, alginate and chitosan) or synthetic polymers (polyethylenimine (PEI) and poly(sodium 4-styrenesulfonate (PSS)). The presence of synthetic polymers on the film increased the electrochemical signal of PcL342-354C up to 100 times. Images by Atomic Force Microscopy (AFM) showed that the immobilized PcL342-354C formed self -assembled nanofibers with diameters ranging from 100 to 200 nm on the polymeric film. By UV -Visible spectroscopy (UV-Vis) it was observed that the ITO/PEI/PSS/PcL342-354C film grows linearly up to the fifth layer, thereafter tending to saturation. X-ray diffraction confirmed the presence on the films of crystalline ITO and amorphous polypeptide phases. In general, the ITO/PEI/PSS/PcL342-354C film characterization proved that this system is an excellent candidate for applications in electrochemical sensors and other biotechnological applications for GMOs and environmental indicators. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 14/02282-6 - Estudo da relação estrutura-atividade de alcalóides derivados da Pilocarpus microphyllus (Rutaceae) com ação contra Schistosoma mansoni
• Beneficiário: Ana Carolina Mafud
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado