High 18F-FDG uptake in PMAH correlated with normal expression of Glut1, HK1, HK2, and HK3

Autor(es)

Cavalcante, Isadora Pontes ^[1] ; Nogueira Zerbini, Maria Claudia ^[2] ; Alencar, Guilherme Asmar ^[1] ; Mariani, Beatriz de Paula ^[1] ; Buchpiguel, Carlos Alberto ^[3] ; Almeida, Madson Queiroz ^{[1, 4]} ; Mendonca, Berenice Bilharinho ^[1] ; Barisson Villares Fragoso, Maria Candida ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Unidade Suprarrenal, Hosp Clin, Disciplina Endocrinol & Metabol, Lab Hormonios & G, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Patol, Sao Paulo - Brazil

^[3] Inst Radiol, Serv Med Nucl & Imagem Mol, Sao Paulo - Brazil

^[4] Inst Canc Sao Paulo ICESP, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACTA RADIOLOGICA; v. 57, n. 3, p. 370-377, MAR 2016.
• Citações Web of Science: 1

Resumo

Background Primary macronodular adrenal hyperplasia (PMAH) is a rare cause of Cushing's syndrome, characterized by functioning adrenal macronodules and variable cortisol production. Recently, we demonstrated a high 18F-FDG uptake in PMAH, an unexpected finding for a benign disorder. Purpose To investigate whether there is a correlation between 18F-FDG high uptake and the expression levels of the glycolytic pathway components GLUT1, HK1, HK2, and HK3 in PMAH. Material and Methods We selected 12 patients undergoing surgery for PMAH who had preoperatively undergone 18F-FDG PET/CT. mRNA and protein expression of the selected genes were evaluated in the adrenal nodules from patients who underwent surgery through quantitative RT-PCR and by immunohistochemistry, respectively. Results SUVmax in PMAH was in the range of 3.3-8.9 and the adrenal size was in the range of 3.5-15cm. A strong correlation between 18F-FDG uptake and largest adrenal diameter was observed in patients with PMAH. However, no correlation between 18F-FDG uptake and GLUT1, HK1, HK2, HK3 mRNA, and protein expression was observed. Conclusion High 18F-FDG uptake is observed in the majority of PMAH cases. However, 18F-FDG uptake in PMAH is independent of the expression levels of GLUT1, HK1, HK2, and HK3. Further investigation is required to elucidate the molecular mechanisms underlying increased 18F-FDG uptake in PMAH. (AU)

• Processo FAPESP: 10/12702-1 - Aspectos clínicos e moleculares da hiperplasia adrenocortical macronodular independente de ACTH em sua forma familial
• Beneficiário: Maria Candida Barisson Villares Fragoso
• Modalidade de apoio: Auxílio à Pesquisa - Regular