Large-scale mitogenomics enables insights into Schizophora (Diptera) radiation and population diversity

Autor(es)

Junqueira, Ana Carolina M. ^[1] ; Azeredo-Espin, Ana Maria L. ^{[2, 3]} ; Paulo, Daniel F. ^{[2, 3]} ; Marinho, Marco Antonio T. ^[4] ; Tomsho, Lynn P. ^[5] ; Drautz-Moses, Daniela I. ^[1] ; Purbojati, Rikky W. ^[1] ; Ratan, Aakrosh ^{[6, 7]} ; Schuster, Stephan C. ^[1]

Afiliação do(s) autor(es)

^[1] Nanyang Technol Univ, Singapore Ctr Environm Life Sci Engn, Singapore 637551 - Singapore

^[2] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083875 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Dept Genet Evolucao & Bioagentes, BR-13083875 Campinas, SP - Brazil

^[4] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil

^[5] Penn State Univ, Dept Biochem & Mol Biol, Ctr Comparat Genom & Bioinformat, University Pk, PA 16802 - USA

^[6] Univ Virginia, Dept Publ Hlth Sci, Charlottesville, VA 22908 - USA

^[7] Univ Virginia, Ctr Publ Hlth Genom, Charlottesville, VA 22908 - USA

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 6, FEB 25 2016.
• Citações Web of Science: 18

Resumo

True flies are insects of the order Diptera and encompass one of the most diverse groups of animals on Earth. Within dipterans, Schizophora represents a recent radiation of insects that was used as a model to develop a pipeline for generating complete mitogenomes using various sequencing platforms and strategies. 91 mitogenomes from 32 different species were sequenced and assembled with high fidelity, using amplicon, whole genome shotgun or single molecule sequencing approaches. Based on the novel mitogenomes, we estimate the origin of Schizophora within the Cretaceous-Paleogene (K-Pg) boundary, about 68.3 Ma. Detailed analyses of the blowfly family (Calliphoridae) place its origin at 22 Ma, concomitant with the radiation of grazing mammals. The emergence of ectoparasitism within calliphorids was dated 6.95 Ma for the screwworm fly and 2.3 Ma for the Australian sheep blowfly. Varying population histories were observed for the blowfly Chrysomya megacephala and the housefly Musca domestica samples in our dataset. Whereas blowflies (n = 50) appear to have undergone selective sweeps and/or severe bottlenecks in the New World, houseflies (n = 14) display variation among populations from different zoogeographical zones and low levels of gene flow. The reported highthroughput mitogenomics approach for insects enables new insights into schizophoran diversity and population history of flies. (AU)

• Processo FAPESP: 09/51723-7 - Genomica mitocondrial com sequenciamento de nova geracao e marcadores moleculares individuais para reconstrucoes filogeneticas em brachycera (diptera)
• Beneficiário: Ana Maria Lima de Azeredo-Espin
• Modalidade de apoio: Auxílio à Pesquisa - Regular
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• Beneficiário: Marco Antonio Tonus Marinho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/56769-2 - Genômica mitocondrial e filogenia molecular do grupo Schizophora (Diptera: Brachycera) utilizando sequenciamento de nova geração
• Beneficiário: Ana Carolina Martins Junqueira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
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• Beneficiário: Daniel Fernando Paulo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica