Melatonin influences on steroidogenic gene expression in the ovary of pinealectomized rats

Autor(es)

Maganhin, Carla C. ^[1] ; Simoes, Ricardo S. ^[2] ; Fuchs, Luiz F. P. ^[2] ; Sasso, Gisela R. S. ^{[3, 4]} ; Simoes, Manuel J. ^{[3, 4]} ; Baracat, Edmund C. ^[2] ; Soares, Jr., Jose M. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Paulista Sch Med, Dept Gynecol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sao Paulo Sch Med, Dept Obstet & Gynecol, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Morphol, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Genet Paulista Sch Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Fertility and Sterility; v. 102, n. 1, p. 291-298, JUL 2014.
• Citações Web of Science: 8

Resumo

Objective: To analyze the expression of genes related to steroidogenesis in the ovary of pinealectomized rats. Design: Experimental research. Setting: University research laboratory. Animal(s): Thirty female adult rats. Intervention(s): Administration of vehicle (GI), pinealectomy with vehicle (GII), or pinealectomy with melatonin replacement (10 mu g/night) for 60 consecutive days (GIII), then euthanasia after 2 months of treatment, ovary collection complementary DNA microarray analyses, confirmatory quantitative reverse-transcriptase polymerase chain reaction analyses, and immunohistochemical analyses for localizing steroidogenesis changes in the ovary. Main Outcome Measure(s): Biologic molecular study followed by immunohistochemical analysis. Result(s): The changes in the expression of CYP11A1, CYP17A1, and CYP19A1 after pinealectomy (GII) compared with control (GI) showed the Cyp17a1 expression level increased in the theca interna and interstitial cells in the GII rats compared with the other groups. Conclusion(s): Melatonin deprivation (pinealectomy) or administration may influence the ovarian CYP17A1 expression and steroidogenesis. (C)2014 by American Society for Reproductive Medicine. (AU)

• Processo FAPESP: 06/60412-7 - Efeitos da melatonina na expressão gênica ovariana em ratas submetidas a pinealectomia
• Beneficiário: Carla Cristina Maganhin
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/54398-4 - Efeitos da melatonina na expressão gênica ovariana em ratas submetidas a pinealectomia
• Beneficiário: José Maria Soares Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular