Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The presence of the NOS3 gene polymorphism for intron 4 mitigates the beneficial effects of exercise training on ambulatory blood pressure monitoring in adults

Texto completo
Autor(es):
Sponton, Carlos H. [1] ; Esposti, Rodrigo [1] ; Rodovalho, Cynara M. [2] ; Ferreira, Maycon J. [1] ; Jarrete, Aline P. [1] ; Anaruma, Chadi P. [1] ; Bacci, Jr., Mauricio [2] ; Zanesco, Angelina [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo State, Lab Cardiovasc Physiol & Exercise Sci, Sao Paulo - Brazil
[2] Univ Sao Paulo State, Inst Biosci, Lab Mol Evolut, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY; v. 306, n. 12, p. H1679-H1691, JUN 15 2014.
Citações Web of Science: 7
Resumo

The number of studies that have evaluated exercise training (ET) and nitric oxide synthase (NOS) 3 gene polymorphisms is scarce. The present study was designed to evaluate the relationship between exercise training and NOS3 polymorphisms at -786T>C, 894G>T, and intron 4b/a on blood pressure (BP) using 24-h ambulatory BP monitoring (ABPM), nitrate/nitrite levels (NOx), and redox state. Eighty-six volunteers (51 +/- 0.6 yr old) were genotyped into nonpolymorphic and polymorphic groups for each of the three positions of NOS3 polymorphisms. Auscultatory BP, ABPM, SOD activity, catalase activity, NOx levels, and malondialdehyde levels were measured. DNA was extracted from leukocytes, and PCR followed by sequencing was applied for genotype analysis. Aerobic ET consisted of 24 sessions for 3 days/wk for 40 min at moderate intensity. This study was performed in a double-blind and crossover format. ET was effective in lowering office BP (systolic BP: 3.2% and diastolic BP: 3%) as well as ABPM (systolic BP: 2% and diastolic BP: 1.3%). Increased SOD and catalase activity (42.6% and 15.1%, respectively) were also observed. The NOS3 polymorphism for intron 4 mitigated the beneficial effect of ET for systolic BP (nonpolymorphic group: -3.0% and polymorphic group: -0.6%) and diastolic BP (nonpolymorphic group: -3.2% and polymorphic group: -0.5%), but it was not associated with NOx level and redox state. Paradoxical responses were found for positions T786-C and G894T for the NOS3 gene. Consistently, the presence of the polymorphism for intron 4 blunted the beneficial effects of ET in middle-aged adults. Possibly, this effect might be as consequence of intron 4 acting as a short intronic repeat RNA controlling endothelial NOS activity epigenetically. (AU)

Processo FAPESP: 11/17437-7 - Efeito do treinamento físico sobre as respostas cardiovasculares e endócrino-metabólicas de mulheres após a menopausa
Beneficiário:Angelina Zanesco
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/00866-0 - Interação dos genótipos/haplótipos para as posições -786T>C, Glu298Asp e intron 4 do gene da eNOS e os efeitos do treinamento físico aeróbio sobre o sistema cardiovascular e a atividade das enzimas antioxidantes em adultos
Beneficiário:Carlos Henrique Grossi Sponton
Linha de fomento: Bolsas no Brasil - Doutorado