Progression of Osteogenic Cell Cultures Grown on Microtopographic Titanium Coated With Calcium Phosphate and Functionalized With a Type I Collagen-Derived Peptide

Autor(es):Mostrar menos -

Pereira, Karina K. Y. ^[1] ; Alves, Olivia C. ^[1] ; Novaes, Jr., Arthur B. ^[1] ; de Oliveira, Fabiola S. ^[1] ; Yi, Ji-Hyun ^[2] ; Zaniquelli, Osvaldo ^[1] ; Wolf-Brandstetter, Cornelia ^[3] ; Scharnweber, Dieter ^[3] ; Variola, Fabio ^[4] ; Nanci, Antonio ^[2] ; Rosa, Adalberto L. ^[1] ; de Oliveira, Paulo T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, BR-14040904 Sao Paulo - Brazil

^[2] Univ Montreal, Lab Study Calcified Tissues & Biomat, Montreal, PQ - Canada

^[3] Tech Univ Dresden, Inst Mat Sci, Max Bergmann Ctr Biomat, Dresden - Germany

^[4] Univ Ottawa, Fac Engn, Ottawa, ON - Canada

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 84, n. 8, p. 1199-1210, AUG 2013.
• Citações Web of Science: 8

Resumo

Background: The functionalization of metallic surfaces aims at promoting the cellular response at the biomaterial-tissue interface. This study investigates the effects of the functionalization of titanium (Ti) microtopography with a calcium phosphate (CaP) coating with and without peptide 15 (P-15), a synthetic peptide analog of the cell-binding domain of collagen I, on the in vitro progression of osteogenic cells. Methods: Sandblasting and acid etching (SBAE; control) Ti microtopography was coated with CaP, enabling the loading of two concentrations of P-15: 20 or 200 mg/mL. A machined Ti was also examined. Rat calvarial osteogenic cells were cultured on Ti disks with the surfaces mentioned above for periods up to 21 days (n = 180 per group). Results: CaP coating exhibited a submicron-scale needle-shaped structure. Although all surfaces were hydrophobic at time zero, functionalization increased hydrophilicity at equilibrium. Microtopographies exhibited a lower proportion of well-spread cells at 4 hours of culture and cells with long cytoplasmic extensions at day 3; modified SBAE supported higher cell viability and larger extracellular osteopontin (OPN) accumulation. For SBAE and modified SBAE, real-time polymerase chain reaction showed the following results: 1) lower levels for runt-related transcription factor 2 at 7 days and for bone sialoprotein at days 7 and 10 as well as higher OPN levels at days 7 and 10 compared to machined Ti; and 2) higher alkaline phosphatase levels at day 10 compared to day 7. At 14 and 21 days, modified SBAE supported higher proportions of red-dye-stained areas (calcium content). Conclusion: Addition of a CaP coating to SBAE Ti by itself may affect key events of in vitro osteogenesis, ultimately resulting in enhanced matrix mineralization; additional P-15 functionalization has only limited synergistic effects. (AU)

• Processo FAPESP: 08/54027-9 - Funcionalizacao de microtopografia de titanio com peptideo sintetico de colageno i (p-15): efeitos sobre diferentes aspectos do desenvolvimento do fenotipo osteogenico in vitro.
• Beneficiário: Paulo Tambasco de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular