Genetic background of novel sequence types of CTX-M-8-and CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae from public wastewater treatment plants in Sao Paulo, Brazil

Autor(es)

Dropa, Milena ^[1] ; Lincopan, Nilton ^{[2, 3]} ; Balsalobre, Livia C. ^[1] ; Oliveira, Danielle E. ^[1] ; Moura, Rodrigo A. ^[2] ; Fernandes, Miriam Rodriguez ^[3] ; da Silva, Quezia Moura ^[2] ; Matte, Glavur R. ^[1] ; Sato, Maria I. Z. ^[4] ; Matte, Maria H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Publ Hlth Lab, Sch Publ Hlth, Ave Dr Arnaldo 715, BR-01146904 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Dept Microbiol, Inst Biomed Sci, Ave Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Sch Pharm, Dept Clin Anal, Ave Prof Lineu Prestes 580, BR-05434070 Sao Paulo, SP - Brazil

^[4] Environm Co Sao Paulo State CETESB, Ave Prof Frederico Hermann Jr 345, BR-05489900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Environmental Science and Pollution Research; v. 23, n. 5, p. 4953-4958, MAR 2016.
• Citações Web of Science: 17

Resumo

The release of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae to the environment is a public health issue worldwide. The aim of this study was to investigate the genetic background of genes encoding ESBLs in wastewater treatment plants (WWTPs) in So Paulo, southeastern Brazil. In 2009, during a local surveillance study, seven ESBL-producing Enterobacteriaceae strains were recovered from five WWTPs and screened for ESBL genes and mobile genetic elements. Multilocus sequence typing (MLST) was carried out, and wild plasmids were transformed into electrocompetent Escherichia coli. S1-PFGE technique was used to verify the presence of high molecular weight plasmids in wild-type strains and in bla (ESBL)-containing E. coli transformants. Strains harbored bla (CTX-M-8), bla (CTX-M-15), and/or bla (SHV-28). Sequencing results showed that bla (CTX-M-8) and bla (CTX-M-15) genes were associated with IS26. MLST revealed new sequence types for E. coli (ST4401, ST4402, ST4403, and ST4445) and Klebsiella pneumoniae (ST1574), except for one K. pneumoniae from ST307 and Enterobacter cloacae from ST131. PCR and S1-PFGE results showed CTX-M-producing E. coli transformants carried heavy plasmids sizing 48.5-209 kb, which belonged to IncI1, IncF, and IncM1 incompatibility groups. This is the first report of CTX-M-8 and SHV-28 enzymes in environmental samples, and the present results demonstrate the plasmid-mediated spread of CTX-M-encoding genes through five WWTPs in So Paulo, Brazil, suggesting WWTPs are hotspots for the transfer of ESBL genes and confirming the urgent need to improve the management of sewage in order to minimize the dissemination of resistance genes to the environment. (AU)

• Processo FAPESP: 10/12841-1 - Identificação e localização de genes de resistência a ²-lactâmicos e tetraciclinas, em cepas clínicas e ambientais de Enterobacteriaceae e Aeromonas hydrophila
• Beneficiário: Maria Helena Matte
• Modalidade de apoio: Auxílio à Pesquisa - Regular