UGP gene expression and UDP-glucose pyrophosphorylase enzymatic activity in grafting annonaceous plant

Autor(es)

Baron, Daniel ^[1] ; Bravo, Juliana P. ^[2] ; Maia, Ivan G. ^[2] ; Pina, Ana ^[3] ; Ferreira, Gisela ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Inst Biociencias, Lab Fisiol Vegetal, Dept Bot, Campus Botucatu S-N, BR-18618970 Botucatu, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Inst Biociencias, Lab Biotecnol & Genet Mol, Dept Genet, Campus Botucatu S-N, BR-18618970 Botucatu, SP - Brazil

^[3] Ctr Invest & Tecnol Agroalimentaria CITA Aragon, Unidad Hortofruticultura, Avda Montanana 930, Zaragoza 500059 - Spain

• Tipo de documento: Artigo Científico
• Fonte: ACTA PHYSIOLOGIAE PLANTARUM; v. 38, n. 3 MAR 2016.
• Citações Web of Science: 7

Resumo

Grafting is commonly used to propagate commercial fruit species to ensure that the genetic characteristics of selected clones are maintained. However, the biochemical and molecular mechanisms involved in the graft incompatibility of woody trees are not well understood. We investigated the effect of grafting in vegetative growth, UDP-glucose pyrophosphorylase expression and activity of Annonaceous grafted plants: atemoya (Annona cherimola Mill. x Annona squamosa L.) `Thompson' grafted onto different rootstocks, araticum-de-terra-fria (Annona emarginata Schltdl. H. Rainer ``var. terra-fria{''}), araticum-mirim (Annona emarginata Schltdl. H. Rainer ``var. mirim{''}) and biribci (Annona mucosa Schltdl. H. Rainer) at different post -grafting times. The growth of atemoya grafted onto araticum-mirim was lower than that of the rootstocks aratictan-de terra-fria and biribci. The results also indicated that grafting alters UGPase gene expression; showing the combination atemoya grafted onto aratictan-de-terra-fria (a compatible union) the higher levels of gene expression during the early stages of grafting development. However, no significant differences were detected in UGPase enzyme activity between the graft combinations. In addition, SDS-PAGE and MALDI-TOF analyses detected similar UGPase amino acid sequences in ungrafted atemoya samples to cherimoya (Annona cherimola Mill.), a female parent of the atemoya hybrid. These findings suggest that expression of the UGPase protein is related to graft compatibility in grafted Annona plants. (AU)

• Processo FAPESP: 13/22036-7 - Estudo da compatibilidade de atemoia (Annona squamosa L. x A. cherimola Mill.) enxertada em araticum-mirim [A. emarginata (Schltdl.) H. Rainer variedade 'Mirim'] e araticum-de-terra-fria [A. emarginata (Schltdl.) H. Rainer variedade 'terra fria']
• Beneficiário: Daniel Baron
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 11/00853-8 - ESTUDO DA COMPATIBILIDADE DE ATEMÓIA (Annona squamosa L. x A. cherimola Mill.) ENXERTADA EM ARATICUM-MIRIM [Annona emarginata (Schltdl.) H. Rainer "VARIEDADE MIRIM] E ARATICUM-DE-TERRA-FRIA [Annona emarginata (Schltdl.) H. Rainer "VARIEDADE TERRA-FRIA"]
• Beneficiário: Daniel Baron
• Modalidade de apoio: Bolsas no Brasil - Doutorado