Incorporation and effects of punicic acid on muscle and adipose tissues of rats

Autor(es)

Pereira de Melo, Illana Louise ^[1] ; de Oliveira e Silva, Ana Mara ^[2] ; Teixeira de Carvalho, Eliane Bonifacio ^[1] ; Yoshime, Luciana Tedesco ^[1] ; Gasparotto Sattler, Jose Augusto ^[1] ; Mancini-Filho, Jorge ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Food & Expt Nutr, Lab Lipids, Fac Pharmaceut Sci, Ave Prof Lineu Prestes, 580 Bloco 14, BR-05508900 Sao Paulo - Brazil

^[2] Univ Fed Sergipe, Biol Sci & Hlth Ctr, Aracaju, Sergipe - Brazil

• Tipo de documento: Artigo Científico
• Fonte: LIPIDS IN HEALTH AND DISEASE; v. 15, FEB 27 2016.
• Citações Web of Science: 3

Resumo

Background: This study evaluated the effect of pomegranate seed oil (PSO) supplementation, rich in punicic acid (55 %/C18: 3-9c, 11 t,13c/CLNA), on the lipid profile and on the biochemical and oxidative parameters in the gastrocnemius muscle and adipose tissues of healthy rats. Linseed oil (LO), rich in linolenic acid (52 %/C18: 3-9c12c15c/LNA) was used for comparison. Methods: Male Wistar rats (n = 56) were distributed in seven groups: control (water); LNA 1 %, 2 % and 4 % (treated with LO); CLNA 1 %, 2 % and 4 % (treated with PSO), po for 40 days. The percentages were compared to the daily feed intake. Fatty acid profile were performed by gas chromatography, antioxidant enzymes activity by spectrophotometer and the adipocytes were isolated by collagenase tissue digestion. Analysis of variance (ANOVA) was applied to check for differences between the groups (control, LNAs and CLNAs) and principal component analysis (PCA) was used to project the groups in the factor-place (PC1 vs PC2) based on the biochemical responses assessed in the study. Results: The fatty acids profile of tissues showed that the LNA percentages were higher in the animals that were fed LO. However, PA was only detected in the adipose tissues. Conjugated linoleic acid (CLA) was present in all the tissues of the animals supplemented with PSO, in a dose dependent manner, and 9c11t-CLA was the predominant isomer. Nevertheless there were no changes in the total weight gain of the animals, the weights of the tissues, and the oxidative stress parameters in the muscle. In addition, there was an increase in the size of the epididymal fat cells in the groups treated with PSO. Principal component analysis (PCA) showed that the CLNAs groups were arranged separately with a cumulative variance of 68.47 %. Conclusions: The results show that PSO can be used as a source of CLAs but that it does not cause changes in body modulation and does not interfere in the antioxidant activity of healthy rats. (AU)

• Processo FAPESP: 09/51891-7 - Avaliação em ratos do efeito dos isômeros conjugados do ácido linoleico (9cis, 11trans e 10trans, 12cis) e do ácido alfa-linolênico (9cis, 11trans, 13cis) sobre o perfil lipídico tecidual e sua influência sobre parâmetros bioquímicos em processos oxidativos
• Beneficiário: Jorge Mancini Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/51890-0 - Avaliacao em ratos do efeito do acido graxo punicio sobre o perfil lipidico tecidual e sua influencia sobre parametros bioquimicos em processos oxidativos
• Beneficiário: Illana Louise Pereira de Melo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/24490-7 - Avaliação química, bioquímica e celular dos isômeros conjugados de ácidos graxos polinsaturados sobre parâmetros bioquímicos em processos oxidativos e inflamatórios
• Beneficiário: Jorge Mancini Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular