Incorporation and effects of punicic acid on muscle and adipose tissues of rats

Author(s)

Pereira de Melo, Illana Louise ^[1] ; de Oliveira e Silva, Ana Mara ^[2] ; Teixeira de Carvalho, Eliane Bonifacio ^[1] ; Yoshime, Luciana Tedesco ^[1] ; Gasparotto Sattler, Jose Augusto ^[1] ; Mancini-Filho, Jorge ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Food & Expt Nutr, Lab Lipids, Fac Pharmaceut Sci, Ave Prof Lineu Prestes, 580 Bloco 14, BR-05508900 Sao Paulo - Brazil

^[2] Univ Fed Sergipe, Biol Sci & Hlth Ctr, Aracaju, Sergipe - Brazil

• Document type: Journal article
• Source: LIPIDS IN HEALTH AND DISEASE; v. 15, FEB 27 2016.
• Web of Science Citations: 3

Abstract

Background: This study evaluated the effect of pomegranate seed oil (PSO) supplementation, rich in punicic acid (55 %/C18: 3-9c, 11 t,13c/CLNA), on the lipid profile and on the biochemical and oxidative parameters in the gastrocnemius muscle and adipose tissues of healthy rats. Linseed oil (LO), rich in linolenic acid (52 %/C18: 3-9c12c15c/LNA) was used for comparison. Methods: Male Wistar rats (n = 56) were distributed in seven groups: control (water); LNA 1 %, 2 % and 4 % (treated with LO); CLNA 1 %, 2 % and 4 % (treated with PSO), po for 40 days. The percentages were compared to the daily feed intake. Fatty acid profile were performed by gas chromatography, antioxidant enzymes activity by spectrophotometer and the adipocytes were isolated by collagenase tissue digestion. Analysis of variance (ANOVA) was applied to check for differences between the groups (control, LNAs and CLNAs) and principal component analysis (PCA) was used to project the groups in the factor-place (PC1 vs PC2) based on the biochemical responses assessed in the study. Results: The fatty acids profile of tissues showed that the LNA percentages were higher in the animals that were fed LO. However, PA was only detected in the adipose tissues. Conjugated linoleic acid (CLA) was present in all the tissues of the animals supplemented with PSO, in a dose dependent manner, and 9c11t-CLA was the predominant isomer. Nevertheless there were no changes in the total weight gain of the animals, the weights of the tissues, and the oxidative stress parameters in the muscle. In addition, there was an increase in the size of the epididymal fat cells in the groups treated with PSO. Principal component analysis (PCA) showed that the CLNAs groups were arranged separately with a cumulative variance of 68.47 %. Conclusions: The results show that PSO can be used as a source of CLAs but that it does not cause changes in body modulation and does not interfere in the antioxidant activity of healthy rats. (AU)

• FAPESP's process: 09/51891-7 - Effect in rats of the isomers of conjugated linoleic acid (9cis, 11trans and 10trans, 12cis) and conjugated a-Iinolenic acid (9cis, 11trans, 13cis) on the tissue lipid profile and its effect on biochemical parameters in oxidative processes
• Grantee: Jorge Mancini Filho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/24490-7 - Evaluation chemistry, biochemistry and cell of conjugated isomers of polyunsaturated fatty acid on biochemical parameters in processes oxidative and inflammatory
• Grantee: Jorge Mancini Filho
• Support Opportunities: Regular Research Grants