Modulation of Candida albicans virulence by bacterial biofilms on titanium surfaces

Autor(es)

Cavalcanti, Yuri Wanderley ^{[1, 2]} ; Wilson, Melanie ^[2] ; Lewis, Michael ^[2] ; Del-Bel-Cury, Altair Antoninha ^[1] ; da Silva, Wander Jose ^[1] ; Williams, David W. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Periodontol & Prosthodont Dept, Piracicaba - Brazil

^[2] Cardiff Univ, Sch Dent, Oral & Biomed Sci, Cardiff CF10 3AX, S Glam - Wales

• Tipo de documento: Artigo Científico
• Fonte: BIOFOULING; v. 32, n. 2, p. 123-134, 2016.
• Citações Web of Science: 10

Resumo

Whilst Candida albicans occurs in peri-implant biofilms, its role in peri-implantitis remains unclear. This study therefore examined the virulence of C. albicans in mixed-species biofilms on titanium surfaces. Biofilms of C. albicans (Ca), C. albicans with streptococci (Streptococcus sanguinis, S. mutans) (Ca-Ss-Sm) and those incorporating Porphyromonas gingivalis (Ca-Pg and Ca-Ss-Sm-Pg) were developed. Expression of C. albicans genes associated with adhesion (ALS1, ALS3, HWP1) and hydrolytic enzymes (SAP2, SAP4, SAP6, PLD1) was measured and hyphal production by C. albicans quantified. Compared with Ca biofilms, significant (p<0.05) up-regulation of ALS3, HWP1, SAP2 and SAP6, and hyphal production occurred in biofilms containing streptococci (Ca-Ss-Sm). In Ca-Pg biofilms, down-regulation of HWP1 and SAP4 expression, with reduced hyphal production occurred. Ca-Ss-Sm-Pg biofilms had increased hyphal proportions and up-regulation of ALS3, SAP2 and SAP6. In conclusion, C. albicans expressed virulence factors in biofilms that could contribute to peri-implantitis, but this was dependent on associated bacterial species. (AU)

• Processo FAPESP: 12/07436-6 - Análise da virulência de biofilmes de Candida albicans desenvolvidos sob diferentes condições
• Beneficiário: Yuri Wanderley Cavalcanti
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto