Modulation of Candida albicans virulence by bacterial biofilms on titanium surfaces

Author(s)

Cavalcanti, Yuri Wanderley ^{[1, 2]} ; Wilson, Melanie ^[2] ; Lewis, Michael ^[2] ; Del-Bel-Cury, Altair Antoninha ^[1] ; da Silva, Wander Jose ^[1] ; Williams, David W. ^[2]

Affiliation

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Periodontol & Prosthodont Dept, Piracicaba - Brazil

^[2] Cardiff Univ, Sch Dent, Oral & Biomed Sci, Cardiff CF10 3AX, S Glam - Wales

• Document type: Journal article
• Source: BIOFOULING; v. 32, n. 2, p. 123-134, 2016.
• Web of Science Citations: 10

Abstract

Whilst Candida albicans occurs in peri-implant biofilms, its role in peri-implantitis remains unclear. This study therefore examined the virulence of C. albicans in mixed-species biofilms on titanium surfaces. Biofilms of C. albicans (Ca), C. albicans with streptococci (Streptococcus sanguinis, S. mutans) (Ca-Ss-Sm) and those incorporating Porphyromonas gingivalis (Ca-Pg and Ca-Ss-Sm-Pg) were developed. Expression of C. albicans genes associated with adhesion (ALS1, ALS3, HWP1) and hydrolytic enzymes (SAP2, SAP4, SAP6, PLD1) was measured and hyphal production by C. albicans quantified. Compared with Ca biofilms, significant (p<0.05) up-regulation of ALS3, HWP1, SAP2 and SAP6, and hyphal production occurred in biofilms containing streptococci (Ca-Ss-Sm). In Ca-Pg biofilms, down-regulation of HWP1 and SAP4 expression, with reduced hyphal production occurred. Ca-Ss-Sm-Pg biofilms had increased hyphal proportions and up-regulation of ALS3, SAP2 and SAP6. In conclusion, C. albicans expressed virulence factors in biofilms that could contribute to peri-implantitis, but this was dependent on associated bacterial species. (AU)

• FAPESP's process: 12/07436-6 - Analysis of the virulence of Candida albicans biofilms developed under different conditions
• Grantee: Yuri Wanderley Cavalcanti
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)