Microbiome of titanium and zirconia dental implants abutments

Autor(es)

do Nascimento, Cassio ^[1] ; Pita, Murillo Sucena ^[1] ; Santos, Emerson de Souza ^[2] ; Monesi, Nadia ^[2] ; Pedrazzi, Vinicius ^[1] ; de Albuquerque Junior, Rubens Ferreira ^{[1, 3]} ; Ribeiro, Ricardo Faria ^[1]

Afiliação do(s) autor(es)

^[1] Fac Dent Ribeirao Preto, Dept Dent Mat & Prosthodont, Mol Diag Lab, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Clin Toxicol & Bromatol Anal, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] McGill Univ, Fac Dent, Strathcona Anat & Dent, 3640 Univ St, Montreal, PQ H3A 2B2 - Canada

• Tipo de documento: Artigo Científico
• Fonte: Dental Materials; v. 32, n. 1, p. 93-101, JAN 2016.
• Citações Web of Science: 20

Resumo

Objectives. This study employed culture-independent molecular techniques to extend the characterization of the microbial diversity of biofilm associated with either titanium or zirconia implant-abutments, including not-yet-cultivated bacteria species, and to identify and quantify species recovered from peri-implantar/periodontal sulci, supragingival biofilm and the internal parts of implants. Probing depth, clinical attachment level, bleeding on probing, and marginal bone level were also evaluated over time and correlated with biofilm formation. Methods. Twenty healthy participants were analyzed. DNA-Checkerboard and 16S-rDNA-Pyrosequencing were used to quantify and determine species identity. Results. 161 bacterial taxa representing 12 different phylotypes were found, of which 25% were non-cultivable. Species common to all sites belonged to genera Fusob a cterium, Prevotella, Actinomyces, Porphyromonas, Veillonella and Streptococcus. While some species were subject specific and detected in most sites, other species were site-specific. Moderate to higher levels of unclassified species were found colonizing titanium-related sites. Pathogenic and nonpathogenic species were detected colonizing oral sites in both materials. Titanium-related sites presented the highest total microbial count and higher counts of pathogenic species. Conclusions. Our results revealed differences regarding microbial diversity and microorganisms counts in oral biofilm associated with titanium or zirconia. The obtained data suggests a possible relation between microbiological findings and clinical outcomes. Significance. Next-generation methods of detection have provided new insights on complex microbiota colonizing different sites of oral cavity. The present study demonstrates relevant differences in the communities and microbial counts colonizing different tested substrates with consequent significant differences in the clinical-outcomes, suggesting a probably different mechanism for specific bacterial adhesion. (C) 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/10442-2 - Avaliação da contaminação peri e intra-implantar de próteses cimentadas sobre conectores de titânio ou zircônia pelas técnicas DNA Checkerboard e 16S rDNAbased PCR.
• Beneficiário: Cássio Do Nascimento
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/12830-0 - Avaliação da contaminação peri e intra-implantar de próteses cimentadas sobre conectores de titânio ou zircônia pelas técnicas DNA checkerboard e 16S rDNAbased PCR
• Beneficiário: Ricardo Faria Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular