The impact of anchored phylogenomics and taxon sampling on phylogenetic inference in narrow-mouthed frogs (Anura, Microhylidae)

Autor(es):Mostrar menos -

Peloso, Pedro L. V. ^{[1, 2]} ; Frost, Darrel R. ^[2] ; Richards, Stephen J. ^[3] ; Rodrigues, Miguel T. ^[4] ; Donnellan, Stephen ^[5] ; Matsui, Masafumi ^[6] ; Raxworthy, Cristopher J. ^[2] ; Biju, S. D. ^[7] ; Lemmon, Emily Moriarty ^[8] ; Lemmon, Alan R. ^[9] ; Wheeler, Ward C. ^[10]

Afiliação do(s) autor(es)

^[1] Amer Museum Nat Hist, Richard Gilder Grad Sch, New York, NY 10024 - USA

^[2] Amer Museum Nat Hist, Div Vertebrate Zool Herpetol, Cent Pk West 79th St, New York, NY 10024 - USA

^[3] S Australian Museum, Herpetol Dept, N Terrace, Adelaide, SA 5000 - Australia

^[4] Univ Sao Paulo, Inst Biociencias, Dept Zool, Rua Matao, Trav 14, 321, Cidade Univ, BR-05422970 Sao Paulo, SP - Brazil

^[5] Univ Adelaide, Ctr Evolutionary Biol & Biodivers, Adelaide, SA 5005 - Australia

^[6] Kyoto Univ, Grad Sch Human & Environm Studies, Sakyo Ku, Kyoto 6068501 - Japan

^[7] Univ Delhi, Systemat Lab, Dept Environm Studies, Delhi 110007 - India

^[8] Florida State Univ, Dept Biol Sci, Tallahassee, FL 32306 - USA

^[9] Florida State Univ, Dept Comp Sci, Dirac Sci Lib, Tallahassee, FL 32306 - USA

^[10] Amer Museum Nat Hist, Div Invertebrate Zool, Cent Pk West 79th St, New York, NY 10024 - USA

• Tipo de documento: Artigo Científico
• Fonte: CLADISTICS; v. 32, n. 2, p. 113-140, APR 2016.
• Citações Web of Science: 54

Resumo

Despite considerable progress in unravelling the phylogenetic relationships of microhylid frogs, relationships among subfamilies remain largely unstable and many genera are not demonstrably monophyletic. Here, we used five alternative combinations of DNA sequence data (ranging from seven loci for 48 taxa to up to 73 loci for as many as 142 taxa) generated using the anchored phylogenomics sequencing method (66 loci, derived from conserved genome regions, for 48 taxa) and Sanger sequencing (seven loci for up to 142 taxa) to tackle this problem. We assess the effects of character sampling, taxon sampling, analytical methods and assumptions in phylogenetic inference of microhylid frogs. The phylogeny of microhylids shows high susceptibility to different analytical methods and datasets used for the analyses. Clades inferred from maximum-likelihood are generally more stable across datasets than those inferred from parsimony. Parsimony trees inferred within a tree-alignment framework are generally better resolved and better supported than those inferred within a similarity-alignment framework, even under the same cost matrix (equally weighted) and same treatment of gaps (as a fifth nucleotide state). We discuss potential causes for these differences in resolution and clade stability among discovery operations. We also highlight the problem that commonly used algorithms for model-based analyses do not explicitly model insertion and deletion events (i.e. gaps are treated as missing data). Our results corroborate the monophyly of Microhylidae and most currently recognized subfamilies but fail to provide support for relationships among subfamilies. Several taxonomic updates are provided, including naming of two new subfamilies, both monotypic. (AU)

• Processo FAPESP: 03/10335-8 - Sistemática e evolução da herpetofauna neotropical
• Beneficiário: Miguel Trefaut Urbano Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/50146-6 - Filogeografia comparada, filogenia, modelagem paleoclimática e taxonomia de répteis e anfíbios neotropicais
• Beneficiário: Miguel Trefaut Urbano Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático