Statistical optimization of protein hydrolysis using mixture design: Development of efficient systems for suppression of lipid accumulation in 3T3-L1 adipocytes

Autor(es)

Soares de Castro, Ruann Janser ^[1] ; Inacio, Rodrigo Fabrizzio ^[2] ; Rodrigues de Oliveira, Alexandre Leite ^[2] ; Sato, Helia Harumi ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Food Engn, Dept Food Sci, 80 Rua Monteiro Lobato, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Anat, Lab Nerve Regenerat, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY; v. 5, p. 17-23, JAN 2016.
• Citações Web of Science: 0

Resumo

Soy protein isolate (SPI), bovine whey protein (BWP) and egg white protein (EWP) were hydrolyzed with the protease Flavourzyme (TM) 500 L, and the interactions of these substrates for the inhibition of the relative lipid accumulation (RLA) in 3T3-L1 preadipocytes during differentiation was studied using a simplex-centroid mixture design. The results indicated that there were synergistic effects for mixtures of non-hydrolyzed and hydrolyzed proteins. The hydrolyzed mixture containing BWP (1/2) and EWP (1/2) at 800 mu g mL(-1) showed increases of up to 220 and 27% in their activities, respectively, compared to the isolated substrates, reaching a maximum RLA suppression of 15.5%. The treatment in which the two-day postconfluent 3T3-L1 preadipocytes received 1200 tg mL(-1) of the mixture containing BWP (1/2) and EWP (1/2) at day zero and every two days afterwards until the end of the experiment at day eight was demonstrated to be more effective, reaching an RLA suppression of 47.9%. The results from the fractionation by ultrafiltration indicated that the non-fractionated sample of BWP (1/2) and EWP (1/2) was the most active for the anti-adipogenic activity and indicated that there is an important contribution of peptide fractions with various molecular sizes in the RLA suppression in 3T3-L1 cells. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/10429-9 - Produção e aplicação de proteases de Aspergillus oryzae na obtenção de peptídeos com atividade biológica
• Beneficiário: Hélia Harumi Sato
• Modalidade de apoio: Auxílio à Pesquisa - Regular