Experimental Calcium Silicate-Based Cement with and without Zirconium Oxide Modulates Fibroblasts Viability

Autor(es)

Camila Slompo ^[1] ; Camila Peres-Buzalaf ^[2] ; Kellen Cristina da Silva Gasque ^[3] ; Carla Andreotti Damante ^[4] ; Ronald Ordinola-Zapata ^[5] ; Marco Antonio Hungaro Duarte ^[6] ; Rodrigo Cardoso de Oliveira ^[7]

Afiliação do(s) autor(es)

^[1] USP - Universidade de São Paulo. Bauru School of Dentistry. Department of Biological Sciences - Brasil

^[2] USC - Universidade do Sagrado Coração. Centro de Ciências da Saúde - Brasil

^[3] USP - Universidade de São Paulo. Bauru School of Dentistry. Department of Biological Sciences - Brasil

^[4] USP - Universidade de São Paulo. Bauru Dental School. Department of Prosthesis and Periodontology - Brasil

^[5] USP - Universidade de São Paulo. Bauru Dental School. Department of Dentistry, Endodontics and Dental Materials - Brasil

^[6] USP - Universidade de São Paulo. Bauru Dental School. Department of Dentistry, Endodontics and Dental Materials - Brasil

^[7] USP - Universidade de São Paulo. Bauru School of Dentistry. Department of Biological Sciences - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 26, n. 6, p. 587-591, 2015-12-00.

Resumo

The aim of this study was to verify whether the use of zirconium oxide as a radiopacifier of an experimental calcium silicate-based cement (WPCZO) leads to cytotoxicity. Fibroblasts were treated with different concentrations (10 mg/mL, 1 mg/mL, and 0.1 mg/mL) of the cements diluted in Dulbecco's modified Eagle's medium (DMEM) for periods of 12, 24, and 48 h. Groups tested were white Portland cement (WPC), white Portland cement with zirconium oxide (WPCZO), and white mineral trioxide aggregate Angelus (MTA). Control group cells were not treated. The cytotoxicity was evaluated through mitochondrial-activity (MTT) and cell-density (crystal violet) assays. All cements showed low cytotoxicity. In general, at the concentration of 10 mg/mL there was an increase in viability of those groups treated with WPC and WPCZO when compared to the control group (p<0.05). A similar profile for the absorbance values was noted among the groups: 10 mg/mL presented an increase in viability compared to the control group. On the other hand, smaller concentrations presented a similar or lower viability compared to the control group, in general. A new dental material composed of calcium silicate-based cement with 20% zirconium oxide as the radiopacifier showed low cytotoxicity as a promising material to be exploited for root-end filling. (AU)

• Processo FAPESP: 09/11585-4 - "Efeitos do cimento Portland puro e associado à duas substâncias radiopacificadoras na proliferação celular de fibroblastos."
• Beneficiário: Camila Slompo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica