Quantum dot effects upon the interaction between porphyrins and phospholipids in cell membrane models

Autor(es)

Parra, Gustavo G. ^{[1, 2]} ; Borissevitch, Galina ^[3] ; Borissevitch, Iouri ^[1] ; Ramos, Ana P. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis, BR-14040901 Sao Paulo - Brazil

^[2] Case Western Reserve Univ, Dept Chem, Cleveland, OH 44106 - USA

^[3] Estacio UNISEB Ctr Univ, BR-14096160 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS; v. 45, n. 3, p. 219-227, APR 2016.
• Citações Web of Science: 2

Resumo

This study employed surface pressure isotherms and spectroscopic techniques to investigate the effect of quantum dots on the interaction between porphyrins and phospholipids using Langmuir monolayers and Langmuir-Blodgett films formed from negatively charged DMPA (the sodium salt of dimyristoyl-sn-glycero-phosphatidyl acid) and zwitterionic DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) as cell membrane models in the presence of 5,10,15,20-tetrakis(4-N-tetradecyl-pyridyl) porphyrin (TMPyP), 5,10,15,20-tetrakis(p-sulfonato-phenyl) porphyrin (TPPS4) and PEG-coated CdSe/ZnS quantum dots (QD). The porphyrins present at the monolayer subphase affected the organization of the lipids at the air/liquid interface, as shown by the changes in the surface pressure-surface area isotherms. QDs enhanced the interaction of TMPyP with DMPA, improving their transference from the liquid monolayers to solid supports. A higher amount of TMPyP was transferred to DMPA-Langmuir-Blodgett films when the QDs were present in the subphase as evidenced by the UV-Vis data. For DPPC the surface effects due to the presence of QDs are less evident. (AU)

• Processo FAPESP: 12/20946-3 - Estudo do crescimento de fosfatos e carbonatos de cálcio sobre matrizes orgânicas depositadas sobre suportes metálicos: obtenção de biomateriais osteoindutores
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