May Sonic Hedgehog proteins be markers for malignancy in uterine smooth muscle tumors?

Autor(es)

Garcia, Natalia ^[1] ; Bozzini, Nilo ^[1] ; Baiocchi, Glauco ^[2] ; da Cunha, Isabela Werneck ^[2] ; Maciel, Gustavo Arantes ^[1] ; Soares Junior, Jose Maria ^[1] ; Soares, Fernando Augusto ^[2] ; Baracat, Edmund Chada ^[1] ; Carvalho, Katia Candido ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Hosp Clin, Lab Mol & Struct Gynecol, Disciplina Ginecol, Fac Med, BR-01245903 Sao Paulo - Brazil

^[2] Hosp AC Camargo Canc Ctr, Int Res Ctr, CIPE, BR-01509010 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: HUMAN PATHOLOGY; v. 50, p. 43-50, APR 2016.
• Citações Web of Science: 3

Resumo

Several studies have demonstrated that the Sonic Hedgehog signaling pathway (SHH) plays an important role in tumorigenesis and cellular differentiation. We analyzed the protein expression of SHH pathway components and evaluated whether their profile could be useful for the diagnosis, prognosis, or prediction of the risk of malignancy for uterine smooth muscle tumors (USMTs). A total of 176 samples (20 myometrium, 119 variants of leiomyoma, and 37 leiomyosarcoma) were evaluated for the protein expression of the SHH signaling components, HHIP1 (SHH inhibitor), and BMP4 (SHE target) by immunohistochemistry. Western blot analysis was performed to verify the specificity of the antibodies. We grouped leiomyoma samples into conventional leiomyomas and unusual leiomyomas that comprise atypical, cellular, mitotically active leiomyomas and uterine smooth muscle tumors of uncertain malignant potential. Immunohistochemical analysis showed that SMO, SUFU, GLIL GLI3, and BMP4 expression gradually increased depending on to the histologic tissue type. The protein expression of SMO, SUFU, and GLI1 was increased in unusual leiomyoma and leiomyosarcoma samples compared to normal myometrium. The inhibitor HHIP1 showed higher expression in myometrium, whereas only negative or basal expression of SMO, SUFU, GLIL and GLI3 was detected in these samples. Strong expression of SHE was associated with poorer overall survival. Our data suggest that the expression of SHH proteins can be useful for evaluating the potential risk of malignancy for USMTs. Moreover, GLI1 and SMO may serve as future therapeutic targets for women with USMTs. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/10098-5 - Via de Sinalização do Sonic Hedghog em leiomioma e leiomiossarcoma uterinos:Estudo da expressão protéica,e transcricional, e avaliação do perfil de metilação dos genes envolvidos na via
• Beneficiário: Kátia Cândido Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular