The fecal virome of South and Central American children with diarrhea includes small circular DNA viral genomes of unknown origin

Autor(es)

Phan, Tung Gia ^{[1, 2]} ; da Costa, Antonio Charlys ^{[1, 3]} ; del Valle Mendoza, Juana ^[4] ; Bucardo-Rivera, Filemon ^{[5, 6]} ; Nordgren, Johan ^[7] ; O'Ryan, Miguel ^[8] ; Deng, Xutao ^[1] ; Delwart, Eric ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Blood Syst Res Inst, San Francisco, CA 94118 - USA

^[2] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94118 - USA

^[3] Univ Sao Paulo, Sch Med, Inst Trop Med, Sao Paulo - Brazil

^[4] Univ Peruana Ciencias Aplicadas UPC, Sch Med, Fac Hlth Sci, Lima - Peru

^[5] Inst Invest Nutr, Lima - Peru

^[6] Univ Leon, Dept Microbiol, Leon - Nicaragua

^[7] Linkoping Univ, Dept Clin & Expt Med, Div Mol Virol, Linkoping - Sweden

^[8] Univ Chile, Fac Med, Microbiol & Mycol Program, Santiago 7 - Chile

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF VIROLOGY; v. 161, n. 4, p. 959-966, APR 2016.
• Citações Web of Science: 16

Resumo

Viral metagenomics of feces collected from 58 Peruvian children with unexplained diarrhea revealed several small circular ssDNA genomes. Two genomes related to sequences previously reported in feces from chimpanzees and other mammals and recently named smacoviruses were characterized and then detected by PCR in 1.7 % (1/58) and 19 % (11/58) of diarrheal samples, respectively. Another three genomes from a distinct small circular ssDNA viral group provisionally called pecoviruses encoded Cap and Rep proteins with < 35 % identity to those in related genomes reported in human, seal, porcine and dromedary feces. Pecovirus DNA was detected in 15.5 % (9/58), 5.9 % (3/51) and 3 % (3/100) of fecal samples from unexplained diarrhea in Peru, Nicaragua and Chile, respectively. Feces containing these ssDNA genomes also contained known human enteric viral pathogens. The cellular origins of these circular ssDNA viruses, whether human cells, ingested plants, animals or fungal foods, or residents of the gut microbiome, are currently unknown. (AU)

• Processo FAPESP: 14/05211-2 - Metagenômica viral em plasma de doadores de sangue em dois bancos de sangue brasileiro
• Beneficiário: Antonio Charlys da Costa
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto
• Processo FAPESP: 12/03417-7 - Caracterização do vírus da dengue pela análise do genoma completo viral em amostras de doadores e receptores de sangue nos estados de Pernambuco e Rio de Janeiro
• Beneficiário: Antonio Charlys da Costa
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto