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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Ultra-Deep Sequencing of HIV-1 near Full-Length and Partial Proviral Genomes Reveals High Genetic Diversity among Brazilian Blood Donors

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Autor(es):
Pessoa, Rodrigo [1] ; Loureiro, Paula [2] ; Lopes, Maria Esther [3] ; Carneiro-Proietti, Anna B. F. [4] ; Sabino, Ester C. [5] ; Busch, Michael P. [6] ; Sanabani, Sabri S. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Med, Clin Lab, Dept Pathol, LIM 03, HC, Sao Paulo - Brazil
[2] Pernambuco State Ctr Hematol & Hemotherapy HEMOPE, Recife, PE - Brazil
[3] Hemorio, Rio De Janeiro - Brazil
[4] Minas Gerais State Ctr Hematol & Hemotherapy HEMO, Belo Horizonte, MG - Brazil
[5] Univ Sao Paulo, Inst Trop Med, Dept Infect Dis, Sao Paulo - Brazil
[6] Blood Syst Res Inst, San Francisco, CA - USA
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 11, n. 3 MAR 31 2016.
Citações Web of Science: 13
Resumo

Background Here, we aimed to gain a comprehensive picture of the HIV-1 diversity in the northeast and southeast part of Brazil. To this end, a high-throughput sequencing-by-synthesis protocol and instrument were used to characterize the near full length (NFLG) and partial HIV-1 proviral genome in 259 HIV-1 infected blood donors at four major blood centers in Brazil: Pro-Sangue foundation (Sao Paulo state (SP), n 51), Hemominas foundation (Minas Gerais state (MG), n 41), Hemope foundation (Recife state (PE), n 96) and Hemorio blood bank (Rio de Janeiro (RJ), n 70). Materials and Methods A total of 259 blood samples were obtained from 195 donors with long-standing infections and 64 donors with a lack of stage information. DNA was extracted from the peripheral blood mononuclear cells (PBMCs) to amplify the HIV-1 NFLGs from five overlapping fragments. The amplicons were molecularly bar-coded, pooled, and sequenced by Illumina paired-end protocol. Results Of the 259 samples studied, 208 (80%) NFLGs and 49 (18.8%) partial fragments were de novo assembled into contiguous sequences and successfully subtyped. Of these 257 samples, 183 (71.2%) were pure subtypes consisting of clade B (n = 167, 65%), C (n = 10, 3.9%), F1 (n = 4, 1.5%), and D (n = 2, 0.7%). Recombinant viruses were detected in 74 (28.8%) samples and consist of unique BF1 (n = 41, 15.9%), BC (n = 7, 2.7%), BCF1 (n = 4, 1.5%), CF1 and CDK (n = 1, 0.4%, each), CRF70\_BF1 (n = 4, 1.5%), CRF71\_BF1 (n = 12, 4.7%), and CRF72\_BF1 (n = 4, 1.5%). Evidence of dual infection was detected in four patients coinfected with the same subtype (n = 3) and distinct subtype (n = 1). Conclusion Based on this work, subtype B appears to be the prevalent subtype followed by a high proportion of intersubtype recombinants that appeared to be arising continually in this country. Our study represents the largest analysis of the viral NFLG ever undertaken worldwide and provides insights into the understanding the genesis of the HIV-1 epidemic in this particular area of South America and informs vaccine design and clinical trials. (AU)

Processo FAPESP: 14/24596-2 - Sequenciamento em larga escala dos genomas do HIV na forma de RNA livre e DNA integrado: comparação entre os subtipos gerados, mutações relacionadas a resistência de drogas e o uso de co-receptores
Beneficiário:Rodrigo Pessoa de Farias
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/12297-2 - Análise dos transcritos de miRNAs, REX e tax em células T no curso da infecção pelo HTLV-1, utilizando sequenciamento de nova geração
Beneficiário:Sabri Saeed Mohamed Ahmed Al-Sanabani
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/11090-5 - Caracterização do vírus da imunodeficiência humana (HIV-1) pela análise do genoma completo viral em amostras de doadores de sangue nos estados de Pernambuco, Minas Gerais, Rio de Janeiro e São Paulo
Beneficiário:Sabri Saeed Mohamed Ahmed Al-Sanabani
Linha de fomento: Auxílio à Pesquisa - Regular