Chelonid herpesvirus 5 in secretions and tumor tissues from green turtles (Chelonia mydas) from Southeastern Brazil: A ten-year study

Autor(es)

Monezi, Telma A. ^{[1, 2]} ; Mehnert, Dolores U. ^[2] ; de Moura, Elisabeth M. M. ^[2] ; Muller, Natascha M. G. ^[2] ; Garrafa, Patricia ^[2] ; Matushima, Eliana R. ^[3] ; Werneck, Max R. ^[4] ; Borella, Maria I. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, BR-05508900 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Ave Prof Lineu Prestes 1374, Cidade Univ, BR-05508900 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Coll Vet Med & Anim Sci, Dept Pathol, BR-05508900 Sao Paulo, SP - Brazil

^[4] Projeto TAMAR ICMBIO, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Microbiology; v. 186, p. 150-156, APR 15 2016.
• Citações Web of Science: 6

Resumo

Fibropapillomatosis (FP), a neoplastic disease characterized by the formation of multiple tumors affecting different species of sea turtles and, most often, the green turtle (Chelonia mydas), is considered one of the major threats to the survival of this species. Recent studies indicate that Chelonid herpesvirus (ChHV5) is the etiological agent of this disease, though its association with anthropogenically altered environments and the immune status of these animals also appears to contribute to disease expression and tumor formation. In this study, tumor biopsy and secretions from green turtles captured off the coast of Sao Paulo State, Brazil, were used in histological and molecular analyses to detect and characterize circulating ChHV5. In 40.9% of cases, the tumor histopathological findings revealed focal ballooning degeneration with intranuclear inclusion bodies, results which are suggestive of viral infection. ChHV5 was detected using polymerase chain reaction (PCR) on the animals' skin, ocular tumor biopsies, and ocular and oral secretions. The analysis of the detected ChHV5 sequences revealed two distinct genetic sequences together. Phylogenetic analysis indicated that Brazilian samples were similar to ChHV5 samples described for the Atlantic phylogeographic group and are therefore part of the same clade as the Gulf of Guinea and Puerto Rico samples. This similarity suggests a possible flow of the virus between these three regions. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 04/13218-5 - Estudo etiologico da fibropapilomatose de tartarugas marinhas da especie chelonia mydas.
• Beneficiário: Eliana Reiko Matushima
• Modalidade de apoio: Auxílio à Pesquisa - Regular