Comparison of Gold CD-R Types as Electrochemical Device and as Platform for Biosensors

Autor(es)

Foguel, Marcos V. ^[1] ; dos Santos, Glauco P. ^[1] ; Ferreira, Antonio A. P. ^[1] ; Magnani, Marina ^[1] ; Mascini, Marcello ^[2] ; Skladal, Petr ^[3] ; Benedetti, Assis V. ^[1] ; Yamanaka, Hideko ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Quim, BR-14800900 Araraquara, SP - Brazil

^[2] Univ Teramo, Fac Biosci & Technol Food Agr & Environm, I-64023 Teramo - Italy

^[3] Masaryk Univ, Dept Biochem, CS-61137 Brno - Czech Republic

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 27, n. 4, p. 650-662, APR 2016.
• Citações Web of Science: 3

Resumo

The construction of gold electrodes using 3 kinds of gold recordable compact discs (CDtrodes) and characterization by electrochemical and spectroscopic techniques are herein reported. Following a surface pretreatment in 0.5 mol L-1 sulfuric acid (H2SO4), the electrochemical experiments were performed in the presence of redox couple ferro/ferricyanide [Fe(CN)6]3-/4-. The immobilization of an oligopeptide bio-inspired by acetylcholinesterase onto the CDtrode was also evaluated. Surface characterization was done by cyclic voltammetry, electrochemical impedance spectroscopy, diffuse reflectance infrared Fourier transform spectroscopy and atomic force microscopy. CDtrodes are known to be of low cost, fast and simple construction. They are suitable for immobilizing biological molecules and could be used as disposable transducer for the construction of biosensors. (AU)

• Processo FAPESP: 08/07729-8 - Desenvolvimento de imunossensor para aflatoxina B1
• Beneficiário: Marcos Vinicius Foguel
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/04663-6 - Imobilização de composto biomimético em eletrodo de ouro
• Beneficiário: Glauco Pilon dos Santos
• Modalidade de apoio: Bolsas no Brasil - Mestrado