Comparison of Gold CD-R Types as Electrochemical Device and as Platform for Biosensors

Author(s)

Foguel, Marcos V. ^[1] ; dos Santos, Glauco P. ^[1] ; Ferreira, Antonio A. P. ^[1] ; Magnani, Marina ^[1] ; Mascini, Marcello ^[2] ; Skladal, Petr ^[3] ; Benedetti, Assis V. ^[1] ; Yamanaka, Hideko ^[1]

Affiliation

^[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Quim, BR-14800900 Araraquara, SP - Brazil

^[2] Univ Teramo, Fac Biosci & Technol Food Agr & Environm, I-64023 Teramo - Italy

^[3] Masaryk Univ, Dept Biochem, CS-61137 Brno - Czech Republic

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 27, n. 4, p. 650-662, APR 2016.
• Web of Science Citations: 3

Abstract

The construction of gold electrodes using 3 kinds of gold recordable compact discs (CDtrodes) and characterization by electrochemical and spectroscopic techniques are herein reported. Following a surface pretreatment in 0.5 mol L-1 sulfuric acid (H2SO4), the electrochemical experiments were performed in the presence of redox couple ferro/ferricyanide [Fe(CN)6]3-/4-. The immobilization of an oligopeptide bio-inspired by acetylcholinesterase onto the CDtrode was also evaluated. Surface characterization was done by cyclic voltammetry, electrochemical impedance spectroscopy, diffuse reflectance infrared Fourier transform spectroscopy and atomic force microscopy. CDtrodes are known to be of low cost, fast and simple construction. They are suitable for immobilizing biological molecules and could be used as disposable transducer for the construction of biosensors. (AU)

• FAPESP's process: 08/07729-8 - Development of immunosensor for aflatoxin B1
• Grantee: Marcos Vinicius Foguel
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 10/04663-6 - Immobilization of biomimetic compound on gold electrode
• Grantee: Glauco Pilon dos Santos
• Support Opportunities: Scholarships in Brazil - Master