Electrochemical capacitive platform for the field-based, label-free detection of DENV

• Grant number: 19/24188-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): January 01, 2020
• Effective date (End): December 31, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Paulo Roberto Bueno
• Grantee: Stephanie Catherine Stoyanow
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated research grant: 17/24839-0 - Nanoelectronics and nanoscale electrochemistry: fundaments and applications, AP.TEM

Abstract

Dengue is the most important arbovirus in the world, being intensified in recent decades. Detecting the disease at an early stage is critical to implementing public policies aimed at reducing the proliferation of the disease in endemic countries and regions where the virus is transmitted. The demand for more information in less time and at a lower cost has spurred new techniques for detecting analyses. Recently, the label-free technique has advanced in the development of biosensors by combining knowledge in materials science, computer design and nanofabrication. Capacitive label-free approaches for molecular diagnostic applications are appealing because of their inherent point-of-care advantages which is particularly important for diagnosing neglected infectious or tropical diseases. In this context, the present work aims the preparation of label-free capacitive immunosensor for identification and quantification of NS1 protein in serological patient samples on conventional gold electrodes comprising ferrocene-tagged peptide and then transfer the system to a surface of gold deposited on silicon wafer (homemade electrodes). The importance of demonstrating the proof-of-principles of electrochemical capacitive methods to real applications in a point-of-care platform open avenues for development not only of miniaturized field-based label-free electrochemical devices but also accurate and highly multiplex able reagent less assays, i.e. where a minimal as possible manipulation of patient samples is required. The detection of Dengue biomarker will be characterized by the electrochemical techniques of cyclic voltammetry (CV) and electrochemical capacitance spectroscopy (ECS).