Antimicrobial peptides: dimerization effects and encapsulation in NRLb biomembrane
| Grant number: | 15/02343-8 |
| Support type: | Regular Research Grants |
| Duration: | June 01, 2015 - May 31, 2017 |
| Field of knowledge: | Biological Sciences - Biochemistry |
| Principal Investigator: | Eduardo Maffud Cilli |
| Grantee: | Eduardo Maffud Cilli |
| Home Institution: | Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil |
| Assoc. researchers: | Norival Alves Santos Filho ; Rondinelli Donizetti Herculano |
Abstract
The biomembrane natural latex (NRL - Natural Rubber Latex), manipulated from the latex obtained by rubber tree Hevea brasiliensis, has shown great potential for application in biomedicine and biomaterials. Due to its biocompatibility and low bounce rate, it has been used for prostheses and grafts, acting as a stimulant of angiogenesis, cell adhesion and extracellular matrix, as well as acting as a physical barrier to infectious agents and controlled release of drugs and extracts. In this work, the incorporation of bioactive peptides on natural biomembrane latex (NRL) and the study of its controlled release will be evaluated. We believe that this study may provide valuable information regarding the controlled release of different peptides and its commercial use. In the second step, we will work with the development of devices "label free" with electroanalytical techniques. These biosensors provide rapid and low-cost methods to assess the stage of the disease and/or efficacy of the treatment. Capacitive redox biosensors have recently been introduced as an electroanalytical assay platform with immediate use and potential applications. In the present work we will synthesized various peptides labeled with a redox probe, in this case ferrocene (fc), to the formation of self-assembled monolayers on metal interfaces to the development of biosensors. As application model will use the detection of C-reactive protein. This analysis is made possible by for antibody (anti-CRP) coupling (carboxyl and amino groups) on the monolayer. Electrochemical techniques such as cyclic voltammetry, electrochemical impedance and capacitance spectroscopy will use in developing the biosensor. We hope in these studies, the development of devices "label free" with high sensitivity and specificity. (AU)