Controlled release of peptides from latex biomembranes and development of capacitive biosensors containing self-assembled peptide

• Grant number: 15/02343-8
• Support type: Regular Research Grants
• Duration: June 01, 2015 - May 31, 2017
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal researcher: Eduardo Maffud Cilli
• Grantee: Eduardo Maffud Cilli
• Home Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Assoc. researchers: Norival Alves Santos Filho ; Rondinelli Donizetti Herculano

Abstract

The biomembrane natural latex (NRL - Natural Rubber Latex), manipulated from the latex obtained by rubber tree Hevea brasiliensis, has shown great potential for application in biomedicine and biomaterials. Due to its biocompatibility and low bounce rate, it has been used for prostheses and grafts, acting as a stimulant of angiogenesis, cell adhesion and extracellular matrix, as well as acting as a physical barrier to infectious agents and controlled release of drugs and extracts. In this work, the incorporation of bioactive peptides on natural biomembrane latex (NRL) and the study of its controlled release will be evaluated. We believe that this study may provide valuable information regarding the controlled release of different peptides and its commercial use. In the second step, we will work with the development of devices "label free" with electroanalytical techniques. These biosensors provide rapid and low-cost methods to assess the stage of the disease and/or efficacy of the treatment. Capacitive redox biosensors have recently been introduced as an electroanalytical assay platform with immediate use and potential applications. In the present work we will synthesized various peptides labeled with a redox probe, in this case ferrocene (fc), to the formation of self-assembled monolayers on metal interfaces to the development of biosensors. As application model will use the detection of C-reactive protein. This analysis is made possible by for antibody (anti-CRP) coupling (carboxyl and amino groups) on the monolayer. Electrochemical techniques such as cyclic voltammetry, electrochemical impedance and capacitance spectroscopy will use in developing the biosensor. We hope in these studies, the development of devices "label free" with high sensitivity and specificity. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MIRANDA, M. C. R.; BORGES, F. A.; BARROS, N. R.; SANTOS FILHO, N. A.; MENDONCA, R. J.; HERCULANO, R. D.; CILLI, E. M. Evaluation of peptides release using a natural rubber latex biomembrane as a carrier. Amino Acids, v. 50, n. 5, p. 503-511, MAY 2018. Web of Science Citations: 3.

PICCOLI, JULIA; HEIN, ROBERT; EL-SAGHEER, AFAF H.; BROWN, TOM; CILLI, EDUARDO M.; BUENO, PAULO R.; DAVIS, JASON J. Redox Capacitive Assaying of C-Reactive Protein at a Peptide Supported Aptamer Interface. Analytical Chemistry, v. 90, n. 5, p. 3005-3008, MAR 6 2018. Web of Science Citations: 13.

MIRANDA, M. C. R.; PREZOTTI, F. G.; BORGES, F. A.; BARROS, N. R.; CURY, B. S. F.; HERCULANO, R. D.; CILLI, E. M. Porosity effects of natural latex (Hevea brasiliensis) on release of compounds for biomedical applications. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, v. 28, n. 18, p. 2117-2130, 2017. Web of Science Citations: 7.

DE BARROS, NATAN ROBERTO; ROMEIRO MIRANDA, MATHEUS CARLOS; BORGES, FELIPE AZEVEDO; DE MENDONCA, RICARDO JOSE; CILLI, EDUARDO MAFFUD; HERCULANO, RONDINELLI DONIZETTI. Oxytocin Sustained Release Using Natural Rubber Latex Membranes. INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS, v. 22, n. 4, p. 435-444, DEC 2016. Web of Science Citations: 12.