MicroRNA Expression Signature Is Altered in the Cardiac Remodeling Induced by High Fat Diets

Autor(es)

Guedes, Elaine Castilho ^[1] ; Franca, Gustavo Starvaggi ^{[2, 3]} ; Lino, Caroline Antunes ^[1] ; Koyama, Fernanda Christtanini ^[2] ; Moreira, Luana do Nascimento ^[1] ; Alexandre, Juliana Gomes ^[1] ; Barreto-Chaves, Maria Luiza M. ^[1] ; Favoretto Galante, Pedro Alexandre ^[2] ; Diniz, Gabriela Placona ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Lab Cell Biol & Funct Anat, Dept Anat, Av Prof Lineu Prestes 2415, BR-05508000 Sao Paulo, SP - Brazil

^[2] Hosp Sirio Libanes, Ctr Mol Oncol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Biochem, Inst Chem, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cellular Physiology; v. 231, n. 8, p. 1771-1783, AUG 2016.
• Citações Web of Science: 11

Resumo

Recent studies have revealed the involvement of microRNAs (miRNAs) in the control of cardiac hypertrophy and myocardial function. In addition, several reports have demonstrated that high fat (HF) diet induces cardiac hypertrophy and remodeling. In the current study, we investigated the effect of diets containing different percentages of fat on the cardiac miRNA expression signature. To address this question, male C57Bl/6 mice were fed with a low fat (LF) diet or two HF diets, containing 45kcal% fat (HF45%) and 60kcal% fat (HF60%) for 10 and 20 weeks. HF60% diet promoted an increase on body weight, fasting glycemia, insulin, leptin, total cholesterol, triglycerides, and induced glucose intolerance. HF feeding promoted cardiac remodeling, as evidenced by increased cardiomyocyte transverse diameter and interstitial fibrosis. RNA sequencing analysis demonstrated that HF feeding induced distinct miRNA expression patterns in the heart. HF45% diet for 10 and 20 weeks changed the abundance of 64 and 26 miRNAs in the heart, respectively. On the other hand, HF60% diet for 10 and 20 weeks altered the abundance of 27 and 88 miRNAs in the heart, respectively. Bioinformatics analysis indicated that insulin signaling pathway was overrepresented in response to HF diet. An inverse correlation was observed between cardiac levels of GLUT4 and miRNA-29c. Similarly, we found an inverse correlation between expression of GSK3 and the expression of miRNA-21a-3p, miRNA-29c-3p, miRNA-144-3p, and miRNA-195a-3p. In addition, miRNA-1 overexpression prevented cardiomyocyte hypertrophy. Taken together, our results revealed differentially expressed miRNA signatures in the heart in response to different HF diets. J. Cell. Physiol. 231: 1771-1783, 2016. (c) 2015 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 13/05742-5 - Efeito da dieta rica em gordura no padrão de expressão de microRNAs no tecido cardíaco
• Beneficiário: Gabriela Placoná Diniz
• Modalidade de apoio: Auxílio à Pesquisa - Regular