Assessing the reproducibility of fractional rates of protein synthesis in muscle tissue measured using the flooding dose technique

Autor(es)

McCarthy, Ian D. ^{[1, 2]} ; Brown, James ^[3]

Afiliação do(s) autor(es)

^[1] Bangor Univ, Coll Nat Sci, Sch Ocean Sci, Askew St, Menai Bridge LL59 5AB, Anglesey - Wales

^[2] Univ Sao Paulo, Inst Oceanog, Lab Manejo Ecol & Conservacao Marinha, Praca Oceanog 191, Cidade Univ, BR-05508120 Sao Paulo, SP - Brazil

^[3] Bangor Univ, Coll Nat Sci, Sch Biol Sci, Deiniol Rd, Bangor LL57 2UW, Gwynedd - Wales

• Tipo de documento: Artigo Científico
• Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY; v. 197, p. 9-15, JUL 2016.
• Citações Web of Science: 0

Resumo

The flooding dose technique of Garlick et al. (1980) has become the main method for measuring tissue and whole-animal rates of protein synthesis in ectotherms. However, single tissue samples are used to determine rates of protein synthesis and no studies have examined the pattern of flooding in large tissues such as the white muscle in fishes, which can comprise up to 55% of the wet body mass of a fish and which is poorly perfused. The present study has examined, for the first time, the patterns of flooding and measured rates of protein synthesis in five different regions of the white muscle in the Arctic charr Salvelinus alpinus ranging in size from 25 g to 1.6 kg following a flooding dose injection of L-{[}H-3]-phenylalanine. The results indicate that the degree of flooding (i.e. free pool specific radioactivity relative to that of the injection solution) and elevation in free phenylalanine concentrations can vary between regions but the calculated fractional rates of protein synthesis were similar in four of the five regions studied. The variability in rates of protein synthesis increased with body size with greater variability observed between regions for fish >1 kg in body mass. For consistency between studies, it is recommended that samples are taken from the epaxial muscle in the region below the dorsal fin when measuring fractional rates of white muscle synthesis in fishes. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 14/21804-3 - Integração da teoria de diferenças individuais consistentes em estudos ecológicos e evolutivos relacionados à mudança ambiental global e local
• Beneficiário: Alexander Turra
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional