Effect of avocado/soybean unsaponifiables on ligature-induced bone loss and bone repair after ligature removal in rats

Autor(es)

Oliveira, G. J. P. L. ^[1] ; Paula, L. G. F. ^[1] ; Souza, J. A. C. ^[1] ; Spin-Neto, R. ^[2] ; Stavropoulos, A. ^[3] ; Marcantonio, R. A. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Sect Periodontol, Dept Diag & Surg, Araraquara Sch Dent, UNESP, Araraquara, SP - Brazil

^[2] Aarhus Univ, Dept Dent, Sect Oral Radiol, Aarhus - Denmark

^[3] Malmo Univ, Dept Periodontol, Fac Odontol, Malmo - Sweden

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PERIODONTAL RESEARCH; v. 51, n. 3, p. 332-341, JUN 2016.
• Citações Web of Science: 4

Resumo

Background and ObjectiveThe aim of this study was to evaluate the effects of administration of avocado/soybean unsaponifiable (ASU), a drug that is commonly used in the treatment of rheumatoid arthritis, on ligature-induced bone loss and bone repair after ligature removal in rats. Material and MethodsEighty-four rats were randomly assigned to four groups of equal size and received a daily gavageof either sterile saline {[}control (CTR)] or ASU (0.6mg/kg), starting 7d before (ASU/-7), on the day of (ASU/0) or 7d after (ASU/+7) periodontitis induction. Periodontitis was induced by placing silk ligatures into the gingival sulcus of the second maxillary molars for 7d; after 7d, the ligatures were removed. Seven rats from each group were sacrificed, 7, 15 or 30d after ligature removal. Bone resorption was evaluated by histomorphometry and micro-computed tomography (micro-CT). Immunohistochemistry was used to evaluate the expression of TRAP, RANKL and alkaline phosphatase (ALP), and quantitative PCR (qPCR) was used to evaluate the levels of interleukin-1beta (Il1), tumor necrosis factor alpha (Tnf), interleukin-6 (Il-6), Rankl and Alp. Statistical analysis was performed using the Shapiro-Wilk test, ANOVA and Tukey's test for normal data, and using the Kruskall-Wallis and Dunnet's tests for non-normal data (p<0.05). ResultsHistomorphometry and micro-CT analysis showed greater bone resorption in the CTR group than in the ASU/0 (15d) and ASU/+7 (7 and 15d) groups. The CTR group also presented with a higher expression of TRAP (15 and 30d) and RANKL (7 and 15d) compared with ASU/0 and ASU/+7 groups. Similarly, qPCR analysis showed higher levels of Rankl and Il1 mRNAs, and lower levels of Alp mRNA, in the CTR group compared with all other groups (for all periods). ConclusionASU exhibited a positive effect on bone repair following ligature-induced periodontitis in rats. (AU)

• Processo FAPESP: 10/13029-9 - Utilização do extrato de óleo insaponificável de abacate e soja na doença periodontal induzida e na osseointegração. Avaliação histológica, radiográfica e imuno-inflamatória em ratos.
• Beneficiário: Rosemary Adriana Chierici Marcantonio
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/17395-2 - Utilização do extrato de óleo insaponificável de abacate e soja na doença periodontal induzida e na osseointegração. Avaliação histológica, radiográfica e imuno-inflamatória em ratos.
• Beneficiário: Guilherme José Pimentel Lopes de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado