Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic ``Aroeira{''} Extract (Myracrodruon urundeuva Allemao)

Autor(es)

Machado, Alessandra Cury ^[1] ; Sartori, Patricia Fernandes ^[1] ; Damante, Carla Andreotti ^[2] ; Dokkedal, Anne Ligia ^[1] ; de Oliveira, Rodrigo Cardoso ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Odontol Bauru, Ciencias Biol, BR-09500900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Odontol Bauru, Protese, BR-09500900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Archives of Biology and Technology; v. 59, 2016.
• Citações Web of Science: 1

Resumo

The aim of this study was to evaluate the effect of ethanolic ``aroeira{''} (Myracrodruon urundeuva) extract on the viability of human gingival fibroblast. For this, fibroblasts (2x10(3) cells/well) were plated in a 96-well plate and incubated for 24 h; the medium (Eagle's medium modified by Dulbecco - DMEM) supplemented with 10% fetal bovine serum was replaced by DMEM with different ethanolic extract concentration (0, 0.1, 1, 10, 100, and 1000 mu g /mL). The fibroblast viability was analyzed after 48,72, and 96 h by the neutral red capture test and violet crystal. The ``aroeira{''} extract, at high concentrations (100 and 1000 mu g/mL) caused decrease in both cellular viability tests (p<0.05). However, dilutions between 0.1 and 10 mu g/mL did not affect the viability of the cells. It was concluded that ``aroeira{''} extract was able to change the gingival fibroblast viability, and this effect was concentration dependent. (AU)

• Processo FAPESP: 11/22243-7 - Efeito do extrato de aroeira na adesão e proliferação de fibroblastos
• Beneficiário: Patricia Fernandes Sartori
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/02026-9 - Avaliação da influência do extrato de aroeira na adesão e proliferação por fibroblatos humanos e expressão de MMP-2 e 9 in vitro
• Beneficiário: Rodrigo Cardoso de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular