Transplantation and survival of mouse inner ear progenitor/stem cells in the organ of Corti after cochleostomy of hearing-impaired guinea pigs: preliminary results

Autor(es)

Barboza, Jr., L. C. M. ^[1] ; Lezirovitz, K. ^[1] ; Zanatta, D. B. ^{[2, 3]} ; Strauss, B. E. ^{[2, 3]} ; Mingroni-Netto, R. C. ^[4] ; Oiticica, J. ^[1] ; Haddad, L. A. ^[4] ; Bento, R. F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Dept Otorrinolaringol LIM32, Hosp Clin, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Med, Lab Genet & Cardiol Mol, Setor Vetores Virais, Inst Coracao, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Fac Med, Ctr Invest Translac Oncol, Lab Vetores Virais, Inst Canc Estado Sao Paulo, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 49, n. 4 2016.
• Citações Web of Science: 2

Resumo

In mammals, damage to sensory receptor cells (hair cells) of the inner ear results in permanent sensorineural hearing loss. Here, we investigated whether postnatal mouse inner ear progenitor/stem cells (mIESCs) are viable after transplantation into the basal turns of neomycin-injured guinea pig cochleas. We also examined the effects of mIESC transplantation on auditory functions. Eight adult female Cavia porcellus guinea pigs (250-350g) were deafened by intratympanic neomycin delivery. After 7 days, the animals were randomly divided in two groups. The study group (n=4) received transplantation of LacZ-positive mIESCs in culture medium into the scala tympani. The control group (n=4) received culture medium only. At 2 weeks after transplantation, functional analyses were performed by auditory brainstem response measurement, and the animals were sacrificed. The presence of mIESCs was evaluated by immunohistochemistry of sections of the cochlea from the study group. Non-parametric tests were used for statistical analysis of the data. Intratympanic neomycin delivery damaged hair cells and increased auditory thresholds prior to cell transplantation. There were no significant differences between auditory brainstem thresholds before and after transplantation in individual guinea pigs. Some mIESCs were observed in all scalae of the basal turns of the injured cochleas, and a proportion of these cells expressed the hair cell marker myosin VIIa. Some transplanted mIESCs engrafted in the cochlear basilar membrane. Our study demonstrates that transplanted cells survived and engrafted in the organ of Corti after cochleostomy. (AU)

• Processo FAPESP: 98/14254-2 - Centro de Estudos do Genoma Humano
• Beneficiário: Mayana Zatz
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 09/09473-3 - Sinalização e diferenciação das células do órgão de Corti: perspectivas para a terapia da surdez
• Beneficiário: Ricardo Ferreira Bento
• Modalidade de apoio: Auxílio à Pesquisa - Regular