SNP discovery from liver transcriptome in the fish Piaractus mesopotamicus

Autor(es)

Mastrochirico-Filho, Vito Antonio ^[1] ; Hata, Milene Elissa ^[1] ; Sato, Lucas Seiti ^[1] ; Jorge, Paulo Henrique ^[1] ; Foresti, Fausto ^[2] ; Rodriguez, Manuel Vera ^[3] ; Martinez, Paulino ^[3] ; Porto-Foresti, Fabio ^[4] ; Hashimoto, Diogo Teruo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Ctr Aquicultura Unesp, Jaboticabal, SP - Brazil

^[2] Univ Estadual Paulista, Dept Morfol, IBB, Botucatu, SP - Brazil

^[3] Univ Santiago de Compostela, Fac Vet, Dept Genet, Lugo - Spain

^[4] Univ Estadual Paulista, Dept Ciencias Biol, FC, Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CONSERVATION GENETICS RESOURCES; v. 8, n. 2, p. 109-114, JUN 2016.
• Citações Web of Science: 4

Resumo

Pacu (Piaractus mesopotamicus) is a Neotropical freshwater fish threatened by overfishing, and one of the species of highest commercial value for aquaculture. Genetic variability analysis through molecular markers is an essential technique to genetic management of the wild and cultivated stocks. The main objective of this study was to identify and validate gene-associated SNPs of the liver transcriptome of pacu. Through genetic analysis in one natural population (Parana River), 32 polymorphic SNPs were successfully genotyped and validated, some of them related to immune system genes. The observed and expected heterozygosity ranged from 0.059 to 0.706 and 0.058 to 0.507, respectively. All loci were in Hardy-Weinberg equilibrium (P > 0.05). Our results showed useful genomic resources for pacu, with applicability in conservation purposes and aquaculture industry by using SNPs markers. (AU)

• Processo FAPESP: 14/03772-7 - Caracterização do transcriptoma e descoberta de SNPs em espécies de Serrasalmidae: subsídios genéticos para a aquicultura
• Beneficiário: Diogo Teruo Hashimoto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/12412-4 - Análise de parentesco e variabilidade genética de pacu (Piaractus mesopotamicus) por meio de marcadores SNPs: subsídios para o melhoramento genético
• Beneficiário: Vito Antonio Mastrochirico Filho
• Modalidade de apoio: Bolsas no Brasil - Mestrado