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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

SNP discovery from liver transcriptome in the fish Piaractus mesopotamicus

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Author(s):
Mastrochirico-Filho, Vito Antonio [1] ; Hata, Milene Elissa [1] ; Sato, Lucas Seiti [1] ; Jorge, Paulo Henrique [1] ; Foresti, Fausto [2] ; Rodriguez, Manuel Vera [3] ; Martinez, Paulino [3] ; Porto-Foresti, Fabio [4] ; Hashimoto, Diogo Teruo [1]
Total Authors: 9
Affiliation:
[1] Univ Estadual Paulista, Ctr Aquicultura Unesp, Jaboticabal, SP - Brazil
[2] Univ Estadual Paulista, Dept Morfol, IBB, Botucatu, SP - Brazil
[3] Univ Santiago de Compostela, Fac Vet, Dept Genet, Lugo - Spain
[4] Univ Estadual Paulista, Dept Ciencias Biol, FC, Bauru, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: CONSERVATION GENETICS RESOURCES; v. 8, n. 2, p. 109-114, JUN 2016.
Web of Science Citations: 4
Abstract

Pacu (Piaractus mesopotamicus) is a Neotropical freshwater fish threatened by overfishing, and one of the species of highest commercial value for aquaculture. Genetic variability analysis through molecular markers is an essential technique to genetic management of the wild and cultivated stocks. The main objective of this study was to identify and validate gene-associated SNPs of the liver transcriptome of pacu. Through genetic analysis in one natural population (Parana River), 32 polymorphic SNPs were successfully genotyped and validated, some of them related to immune system genes. The observed and expected heterozygosity ranged from 0.059 to 0.706 and 0.058 to 0.507, respectively. All loci were in Hardy-Weinberg equilibrium (P > 0.05). Our results showed useful genomic resources for pacu, with applicability in conservation purposes and aquaculture industry by using SNPs markers. (AU)

FAPESP's process: 14/03772-7 - Transcriptome characterization and SNPs discovery in species of Serrasalmidae: genetic subsidies for aquaculture
Grantee:Diogo Teruo Hashimoto
Support Opportunities: Regular Research Grants
FAPESP's process: 14/12412-4 - Analysis of kinship degree and genetic variability of pacu (Piaractus mesopotamicus) through SNPs markers: tools for genetic improvement
Grantee:Vito Antonio Mastrochirico Filho
Support Opportunities: Scholarships in Brazil - Master