Biochemical Modulation by Carbon and Nitrogen Addition in Cultures of Dictyota menstrualis (Dictyotales, Phaeophyceae) to Generate Oil-based Bioproducts

Autor(es)

Martins, Aline P. ^[1] ; Yokoya, Nair S. ^[2] ; Colepicolo, Pio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Bioquim, Inst Quim, Caixa Postal 26077, BR-05599970 Sao Paulo, SP - Brazil

^[2] Secretaria Meio Ambiente Estado Sao Paulo, Inst Bot, Nucleo Pesquisa Ficol, Ave Miguel Estefano 3687, BR-04301012 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MARINE BIOTECHNOLOGY; v. 18, n. 3, p. 314-326, JUN 2016.
• Citações Web of Science: 4

Resumo

Dictyota menstrualis (Hoyt) Schnetter, Horning \& Weber-Peukert (Dictyotales, Phaeophyceae) was studied for the production of oil-based bioproducts and co-products. Experiments were performed to evaluate the effect of carbon dioxide (CO2) concentration, under nitrogen (NO3-) limiting and saturation conditions, on growth rate (GR), photosynthesis, as well as nitrate reductase (NR), carbonic anhydrase (CA), and Rubisco activities. In addition, the biochemical composition of D. menstrualis under these conditions was estimated. GR, protein content, and N content in D. menstrualis were higher in treatments containing NO3-, irrespective of CO2 addition. However, when CO2 was added to medium saturated with NO3-, values of maximum photosynthesis, Rubisco, and NR activity, as well as total soluble carbohydrates and lipids, were increased. CA activity did not vary under the different treatments. The fatty acid profile of D. menstrualis was characterized by a high content of polyunsaturated fatty acids, especially the omega-3 fatty acids, making it a possible candidate for nutraceutical use. In addition, this species presented high GR, photosynthetic rate, and fatty acid content, highlighting its economic importance and the possibility of different biotechnological applications. (AU)

• Processo FAPESP: 12/19844-1 - Utilização de Ulva lactuca (Ulvophyceae, Chlorophyta) para a produção de biocombustível e remoção de nutrientes da água do mar
• Beneficiário: Aline Paternostro Martins
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/50193-1 - Estudos de bioprospecção de macroalgas marinhas, uso da biomassa algal como fonte de novos fármacos e bioativos economicamente viáveis e sua aplicação na remediação de áreas impactadas. (Biodiversidade marinha)
• Beneficiário: Pio Colepicolo Neto
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático