Antigenotoxic and Antioxidant Properties of Solanum cernuum and Its Alkaloid, Cernumidine

Autor(es)

Damasceno, Jaqueline Lopes ^[1] ; de Oliveira, Pollyanna Francielli ^[1] ; Miranda, Mariza Abreu ^[2] ; Lima, Marina ^[1] ; Bastos, Jairo Kenupp ^[2] ; Tavares, Denise Crispim ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, Ave Dr Armando Salles de Oliveira, 201 Parque Univ, BR-14404600 Franca, SP - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ave Bandeirantes, S-N Monte Alegre, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOLOGICAL & PHARMACEUTICAL BULLETIN; v. 39, n. 6, p. 920-926, JUN 2016.
• Citações Web of Science: 2

Resumo

Solanum cernuum YELL. has been used extensively for the treatment of urinary disorders, gonorrhea and skin infections; cernumidine is a major component of S. cernuum (SC) hydroalcoholic extract. The micronucleus test in V79 cells was used to evaluate the genotoxic and antigenotoxic potential of SC and cernumidine. For antigenotoxicity assessment, methyl methanesulfonate (MMS, 44 mu g/mL) and hydrogen peroxide (H2O2, 3.5 mu g/mL) were added as inducers of chromosome damage. Antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. Significantly higher frequencies of micronuclei were observed in cell cultures treated with SC concentrations of 160 and 320 mu g/mL in comparison with the negative control, demonstrating a genotoxic effect. There was no significant difference in the frequency of micronuclei between cell cultures treated with a combination of SC and MMS and those treated only with MMS. On the other hand, a significant reduction in the frequency of micronuclei was observed for V79 cells treated with SC or cernumidine plus H2O2 compared to those treated only with H2O2. Furthermore, SC and cernumidine were able to scavenge free radicals in the DPPH assay. Thus, the protective effect of SC and cernumidine against H2O2 can be attributed to antioxidant activity. (AU)

• Processo FAPESP: 12/00715-7 - Solanum cernuum Vell: estudo fitoquímico, avaliação das atividades antiulcerogênica, antimicrobiana, citotóxica e obtenção de extrato seco por spray dryer
• Beneficiário: Mariza Abreu Miranda
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/05732-4 - Estudo do potencial citotóxico e genotóxico do extrato de Solanum lycocarpum e dos seus compostos majoritários e sua influência sobre a genotoxicidade induzida por diferentes mutágenos
• Beneficiário: Denise Crispim Tavares
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/07946-4 - Estudo do potencial genotóxico e antigenotóxico do extrato de Solanum cernuum em células de mamíferos in vivo e in vitro
• Beneficiário: Jaqueline Lopes Damasceno
• Modalidade de apoio: Bolsas no Brasil - Mestrado