Antigenotoxic and Antioxidant Properties of Solanum cernuum and Its Alkaloid, Cernumidine

Author(s)

Damasceno, Jaqueline Lopes ^[1] ; de Oliveira, Pollyanna Francielli ^[1] ; Miranda, Mariza Abreu ^[2] ; Lima, Marina ^[1] ; Bastos, Jairo Kenupp ^[2] ; Tavares, Denise Crispim ^[1]

Affiliation

^[1] Univ Franca, Ave Dr Armando Salles de Oliveira, 201 Parque Univ, BR-14404600 Franca, SP - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ave Bandeirantes, S-N Monte Alegre, BR-14040901 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: BIOLOGICAL & PHARMACEUTICAL BULLETIN; v. 39, n. 6, p. 920-926, JUN 2016.
• Web of Science Citations: 2

Abstract

Solanum cernuum YELL. has been used extensively for the treatment of urinary disorders, gonorrhea and skin infections; cernumidine is a major component of S. cernuum (SC) hydroalcoholic extract. The micronucleus test in V79 cells was used to evaluate the genotoxic and antigenotoxic potential of SC and cernumidine. For antigenotoxicity assessment, methyl methanesulfonate (MMS, 44 mu g/mL) and hydrogen peroxide (H2O2, 3.5 mu g/mL) were added as inducers of chromosome damage. Antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. Significantly higher frequencies of micronuclei were observed in cell cultures treated with SC concentrations of 160 and 320 mu g/mL in comparison with the negative control, demonstrating a genotoxic effect. There was no significant difference in the frequency of micronuclei between cell cultures treated with a combination of SC and MMS and those treated only with MMS. On the other hand, a significant reduction in the frequency of micronuclei was observed for V79 cells treated with SC or cernumidine plus H2O2 compared to those treated only with H2O2. Furthermore, SC and cernumidine were able to scavenge free radicals in the DPPH assay. Thus, the protective effect of SC and cernumidine against H2O2 can be attributed to antioxidant activity. (AU)

• FAPESP's process: 12/00715-7 - Solanum cernuum Vell: Phytochemical study, evaluation of antiulcerogenic, antimicrobial, cytotoxic activities and attainment of dry extract by spray dryer
• Grantee: Mariza Abreu Miranda
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/05732-4 - Study of cytotoxic and genotoxic potential of Solanum lycocarpum and their majoritary compounds and their influence on genotoxicity induced by different mutagens
• Grantee: Denise Crispim Tavares Barbosa
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/07946-4 - Study of genotoxic and antigenotoixc potential of Solanum cernuum extract in in vivo and in vitro mammalian cells
• Grantee: Jaqueline Lopes Damasceno
• Support Opportunities: Scholarships in Brazil - Master