Evaluation of citotoxicity, genotoxicity, antigenotoxicity and gene expression of TP53 and Ephx2 in rats treated with Caryocar villosum

• Grant number: 09/15692-0
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): April 01, 2010
• Effective date (End): January 31, 2013
• Field of knowledge: Biological Sciences - Genetics
• Principal Investigator: Maria de Lourdes Pires Bianchi
• Grantee: Mara Ribeiro de Almeida
• Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 05/59552-6 - Integrated evaluation of the stability and functional properties of natural pigments of foods, AP.TEM

Abstract

Since 1994 when was established the Research Group Food-Drug Interaction registered at CNPq, our goal has been investigations of the dietary bioactive compounds in the protection of the adverse effects induced by chemotherapy in vivo models. Currently, we cooperate in the thematic project supported by FAPESP "Integrated assessment of the stability and functional properties of food natural pigments", coordinated by Profª. Dr. Adriana Zerlotti Mercadante from FEA-UNICAMP. One of the goals of the thematic is the identification of pigments and extracts from fruits of the Amazon region, in addition to the investigation of their biological properties, to its application in industries of food, pharmaceutical and cosmetics.The diet is involved in several important cellular signaling pathways, as activation/detoxification of carcinogens, DNA repair and apoptosis. The role of diet as a key factor in determining the genomic instability is more important than previously thought and, therefore, the assessment of genotoxic and antigenotoxic potential of the dietary components has become a critically important event in promoting health. Caryocar villosum (Aubl.) Pers. (contry name: Piquiá) is a native plant of the Amazon rainforest. The piquiá fruits pulp are rich sources in ²-carotene, flavonoids and phenolic compounds and have a high antioxidant activity. Due to absence of data in the literature on the biological activity of the piquiá fruit extract, this study aims to evaluate the possible effects of the Caryocar villosum fruit extract through of genomic instability analysis (micronuclei and comet), biochemical changes (glutathione and lipid peroxidation) and gene expression (genes TP53 and EPHX2) in animals treated with the fruit extract associated or not with chemotherapeutic drug.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALMEIDA, MARA RIBEIRO; AISSA, ALEXANDRE FERRO; URSULA HERMOGENES GOMES, TARSILA DAYSY; CASTANIA DARIN, JOANA D'ARC; CHISTE, RENAN CAMPOS; MERCADANTE, ADRIANA ZERLOTTI; GREGGI ANTUNES, LUSANIA MARIA; PIRES BIANCHI, MARIA LOURDES. In Vivo Genotoxicity and Oxidative Stress Evaluation of an Ethanolic Extract from Piquia (Caryocar villosum) Pulp. JOURNAL OF MEDICINAL FOOD, v. 16, n. 3, p. 268-271, MAR 2013. Web of Science Citations: 5.

ALMEIDA, MARA RIBEIRO; CASTANIA DARIN, JOANA D'ARC; HERNANDES, LIVIA CRISTINA; AISSA, ALEXANDRE FERRO; CHISTE, RENAN CAMPOS; MERCADANTE, ADRIANA ZERLOTTI; GREGGI ANTUNES, LUSANIA MARIA; PIRES BIANCHI, MARIA LOURDES. Antigenotoxic Effects of Piquia (Caryocar villosum) in Multiple Rat Organs. PLANT FOODS FOR HUMAN NUTRITION, v. 67, n. 2, p. 171-177, JUN 2012. Web of Science Citations: 15.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARA RIBEIRO DE ALMEIDA. Avaliação da citotoxicidade, genotoxicidade, antigenotoxicidade e expressão dos genes Tp53 e Ephx2 em ratos tratados com Caryocar villosum. 2013. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto Ribeirão Preto.