In vitro and in vivo activities of guajiru fruit (Chrysobalanus icaco L.) in oxidative stress, DNA damage and inflammation biomarkers

• Grant number: 11/21471-6
• Support type: Scholarships in Brazil - Doctorate (Direct)
• Effective date (Start): April 01, 2012
• Effective date (End): September 30, 2016
• Field of knowledge: Health Sciences - Nutrition
• Principal Investigator: Lusânia Maria Greggi Antunes
• Grantee: Vinicius de Paula Venancio
• Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Chrysobalanus icaco or guajiru is an amazonian plant whose leaf extract is widely used on Folk Medicine as hypoglycemic, diuretic, antiangiogenic and antioxidant. The guajiru pulp, however, is still understudied. Thanks to the presence of anthocyanins in this fruit, it's possible that it can have antioxidant and functional properties. Furthermore, it is not known if the same leaf effects can be obtained in vivo when using this fruit. This project aimed at studying antigenotoxicity, genotoxicity, antimutagenicity, mutagenicity, the oxidative stress and the gene expression by an in vivo experimental design, of lyophilized guajiru pulp. Male Wistar rats will be treated by gavage with three doses of C. icaco pulp for 14 consecutive days. On 14th day, the animals will receive doxorubicin 15 mg kg-1 b.w. (i.p.) or 0.9% NaCl (i.p.). 24h after the induction, all animals will be euthanized to obtain blood, bone marrow, kidney, liver, heart and brain samples. The comet assay will be performed on blood and organs to evaluate the genotoxicity and antigenotoxicity. To analyze the mutagenicity and antimutagenicity, the micronuclei assay will be performed on bone marrow and peripheral blood. For the oxidative stress, the following parameters will be evaluated: malondialdehyde, glutathione and catalase (on organs) and oxidative burst (on blood). Finally, the gene expression of SOD-1 and COX-2 will be performed on rat liver, kidney and heart, by real-time PCR. At the same time, at FEA-UNICAMP, the guajiru pulp will be characterized by de evaluation of the major bioactive compounds (phenolic compounds, carotenoids, ascorbic acid, tocopherols). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VENANCIO, VINICIUS PAULA; ALMEIDA, MARA RIBEIRO; GREGGI ANTUNES, LUSANIA MARIA. Cocoplum (Chrysobalanus icaco L.) decreases doxorubicin-induced DNA damage and downregulates Gadd45a, Il-1 beta, and Tnf-alpha in vivo. Food Research International, v. 105, p. 996-1002, MAR 2018. Web of Science Citations: 1.

VENANCIO, VINICIUS PAULA; MARQUES, MARCELLA CAMARGO; ALMEIDA, MARA RIBEIRO; BARROS MARIUTTI, LILIAN REGINA; DE OLIVEIRA SOUZA, VANESSA CRISTINA; BARBOSA, JR., FERNANDO; PIRES BIANCHI, MARIA LOURDES; MARZOCCHI-MACHADO, CLENI MARA; MERCADANTE, ADRIANA ZERLOTTI; GREGGI ANTUNES, LUSANIA MARIA. Chrysobalanus icaco L. fruits inhibit NADPH oxidase complex and protect DNA against doxorubicin-induced damage in Wistar male rats. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 79, n. 20, p. 885-893, 2016. Web of Science Citations: 8.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VENANCIO, Vinicius de Paula. In vitro and in vivo activities of guajiru fruit (Chrysobalanus icaco L.) in oxidative stress, DNA damage, and inflammation biomarkers. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto Ribeirão Preto.