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Antigenotoxic activity of compounds in diet and its effects in the expression of genes in response to oxidative stress


In the last 15 years, our research group has done studies with compounds in the diet. The goals have been the investigation of possible antimutagenic and cytoprotective activities of carotenoids, phenolics and vitamins. The idea of combining micronutrients, mainly antioxidants, for chemoprevention seeks an increasing of their effectiveness, the decline in the use of individual doses and possible reduction of toxicity of drugs. In literature there is still a lack of studies on the synergistic or antagonistic interactions of natural pigments isolated from the diet, as chlorophylls and carotenoids, and their effects on living organisms, since the available data was obtained in in vitro systems. Therefore, this study aims to assessing the potential cytotoxic, genotoxic, mutagenic and protective effects of the isolated natural pigments, chlorophyll "b" and lutein as well as the effects of their mixture. For this, it will be used the tests recommended by international regulatory agencies for assessment of mutagenicity such as micronucleus test in bone marrow cells, peripheral blood and the comet assay in peripheral blood, kidney and liver cells of mice and in human HepG2 line. For the verification of antimutagenic activity of pigments, it is recommend the use of an agent recognized mutagenic. In this case, we rely on the experience of our laboratory in investigations about the damage caused by the chemotherapic cisplatin (cDDP). This study also aims to investigate the effects of these natural pigments in the expression of genes in response to oxidative stress also in liver and kidney cells of mice using tools of molecular biology like the polymerase chain reaction (PCR array) system, and the protein expression profile in HepG2 cells. Among the 96 genes that will be evaluated there are housekeeping genes, genes involved in reactive oxygen species metabolism, antioxidants genes and oxygen transporters. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SERPELONI, JULIANA MARA; BATISTA, BRUNO LEMOS; FRIEDMANN ANGELI, JOSE PEDRO; MAZZARON BARCELOS, GUSTAVO RAFAEL; PIRES BIANCHI, MARIA DE LOURDES; BARBOSA, JR., FERNANDO; GREGGI ANTUNES, LUSANIA MARIA. Antigenotoxic Properties of Chlorophyll b Against Cisplatin-Induced DNA Damage and its Relationship with Distribution of Platinum and Magnesium In Vivo. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 76, n. 6, p. 345-353, . (05/59552-6, 08/06793-4, 10/05096-8)
SERPELONI, JULIANA MARA; GROTTO, DENISE; AISSA, ALEXANDRE FERRO; MERCADANTE, ADRIANA ZERLOTTI; PIRES BIANCHI, MARIA DE LOURDES; GREGGI ANTUNES, LUSANIA MARIA. An evaluation, using the comet assay and the micronucleus test, of the antigenotoxic effects of chlorophyll b in mice. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 725, n. 1-2, p. 50-56, . (10/05096-8, 05/59552-6, 08/06793-4)
SERPELONI, JULIANA MARA; DE SYLLOS COLUS, ILCE MARA; DE OLIVEIRA, FABIOLA SINGARETTI; AISSA, ALEXANDRE FERRO; MERCADANTE, ADRIANA ZERLOTTI; PIRES BIANCHI, MARIA LOURDES; GREGGI ANTUNES, LUSANIA MARIA. Diet carotenoid lutein modulates the expression of genes related to oxygen transporters and decreases DNA damage and oxidative stress in mice. Food and Chemical Toxicology, v. 70, p. 205-213, . (10/05096-8, 05/59552-6, 08/06793-4)

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