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Antigenotoxic activity of compounds in the diet and its effect in the expression of genes in response to oxidative stress

Grant number: 08/06793-4
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2009
Effective date (End): February 29, 2012
Field of knowledge:Health Sciences - Nutrition - Nutrition Biochemistry
Principal Investigator:Lusânia Maria Greggi Antunes
Grantee:Juliana Mara Serpeloni
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:05/59552-6 - Integrated evaluation of the stability and functional properties of natural pigments of foods, AP.TEM

Abstract

In the last 10 years, our research group has done studies with compounds in the diet. The goals have been the investigation of possible antimutagenic and cytoprotective activities of carotenoids, phenolics and vitamins. The idea of combining micronutrients, mainly antioxidants, for chemoprevention seeks an increasing of their effectiveness, the decline in the use of individual doses and possible reduction of toxicity of drugs. In literature there is still a lack of studies on the synergistic or antagonistic interactions of natural pigments isolated from the diet, as chlorophylls and carotenoids, and their effects on living organisms, since the available data was obtained in in vitro systems. Therefore, this study aims to contribute with the research project "Integrated evaluation of the stability and functional properties of natural pigments from foods", by assessing the potential cytotoxic, genotoxic, mutagenic and protective effects of the isolated natural pigments, chlorophyll "b" and lutein as well as the effects of their mixture. For this, it will be used the tests recommended by international regulatory agencies for assessment of mutagenicity such as micronucleus test in bone marrow cells and peripheral blood and the comet assay in peripheral blood, kidney and liver cells of mice. For the verification of antimutagenic activity of pigments, it is recommend the use of an agent recognized mutagenic. In this case, with the experience of our laboratory in investigations about the damage caused by chemotherapy cisplatin (cDDP), this will be the inducer selected for the tests of antimutagenicity. This study also aims to investigate the effects of these natural pigments in the expression of genes in response to oxidative stress also in liver and kidney cells of mice using tools of molecular biology like the polymerase chain reaction (PCR array) system. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SERPELONI, JULIANA MARA; DE SYLLOS COLUS, ILCE MARA; DE OLIVEIRA, FABIOLA SINGARETTI; AISSA, ALEXANDRE FERRO; MERCADANTE, ADRIANA ZERLOTTI; PIRES BIANCHI, MARIA LOURDES; GREGGI ANTUNES, LUSANIA MARIA. Diet carotenoid lutein modulates the expression of genes related to oxygen transporters and decreases DNA damage and oxidative stress in mice. Food and Chemical Toxicology, v. 70, p. 205-213, AUG 2014. Web of Science Citations: 9.
SERPELONI, JULIANA MARA; BATISTA, BRUNO LEMOS; FRIEDMANN ANGELI, JOSE PEDRO; MAZZARON BARCELOS, GUSTAVO RAFAEL; PIRES BIANCHI, MARIA DE LOURDES; BARBOSA, JR., FERNANDO; GREGGI ANTUNES, LUSANIA MARIA. Antigenotoxic Properties of Chlorophyll b Against Cisplatin-Induced DNA Damage and its Relationship with Distribution of Platinum and Magnesium In Vivo. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 76, n. 6, p. 345-353, MAR 1 2013. Web of Science Citations: 0.
SERPELONI, JULIANA MARA; GROTTO, DENISE; AISSA, ALEXANDRE FERRO; MERCADANTE, ADRIANA ZERLOTTI; PIRES BIANCHI, MARIA DE LOURDES; GREGGI ANTUNES, LUSANIA MARIA. An evaluation, using the comet assay and the micronucleus test, of the antigenotoxic effects of chlorophyll b in mice. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 725, n. 1-2, p. 50-56, OCT 9 2011. Web of Science Citations: 12.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SERPELONI, Juliana Mara. Atividade antigenotóxica de compostos da dieta e sua influência na expressão de genes de resposta ao estresse oxidativo. 2012. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto Ribeirão Preto.

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