Titanium scaffold osteogenesis in healthy and osteoporotic rats is improved by the use of low-level laser therapy (GaAlAs)

Autor(es)

Reis de Vasconcellos, Luana Marotta ^[1] ; Moreira Barbara, Mary Anne ^[1] ; Rovai, Emanuel da Silva ^[2] ; Franca, Mariana de Oliveira ^[1] ; Ebrahim, Zahra Fernandes ^[1] ; Oliveira de Vasconcellos, Luis Gustavo ^[3] ; Porto, Camila Deco ^[1] ; Alves Cairo, Carlos Alberto ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Ave Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent, Div Periodont, Dept Stomatol, Ave Lineu Prestes 2227, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Estadual Paulista UNESP, Inst Sci & Technol, Dept Prosthodont & Dent Mat, Ave Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[4] CTA, Air & Space Inst, Div Mat, Praca Mal Ar Eduardo Gomes 14, BR-12904000 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 31, n. 5, p. 899-905, JUL 2016.
• Citações Web of Science: 4

Resumo

The present study aimed to assess the effects of low-level laser therapy (GaAlAs) on the bone repair process within titanium scaffolds in the femurs of healthy and osteoporotic rats. Fifty-six rats were divided into four groups: group Sh: SHAM animals that received scaffolds; group LSh: SHAM animals that received scaffolds and were subjected to laser therapy; group OV: ovarietomized (OVX) animals that received scaffolds; and group LOV: OVX animals that received scaffolds and were subjected to laser therapy. Thirty days following ovariectomy or sham surgery, scaffolds were implanted in the left femurs of all animals in the study. Immediately after opening the surgical site, the inner part of the surgical cavity was stimulated with low-level laser (GaAlAs). In addition to this procedure, the laser group was also subjected to sessions of low-level laser therapy (LLLT) at 48-h intervals, with the first session performed immediately after surgery. The rats were sacrificed at 2 and 6 weeks, time in which femur fragments were submitted for histological and histomorphometric examination, and skin tissue above the scaffold was submitted to histological analysis. At the end of the study, greater bone formation was observed in the animals submitted to LLLT. At 2 and 6 weeks, statistically significant differences were observed between LSh and Sh groups (p = 0.009 and 0.0001) and LOV and OV (p = 0.0001 and 0.0001), respectively. No statistical difference was observed when assessing the estrogen variable. On the basis of our methodology and results, we conclude that LLLT improves and accelerates bone repair within titanium scaffolds in both ovariectomized and healthy rats, when compared to animals not subjected to radiation. (AU)

• Processo FAPESP: 08/09867-9 - Influência da superfície porosa e da laserterapia na osseointegração de implantes e na cicatrização do tecido cutâneo, em ratas osteopênicas
• Beneficiário: Zahra Fernandes Ebrahim
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 08/05619-0 - Influência da superfície porosa e da laserterapia na osseointegração de implantes e na cicatrização de tecido cutâneo, em ratas osteopênicas
• Beneficiário: Mariana de Oliveira França
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica