Behavioral, Ventilatory and Thermoregulatory Responses to Hypercapnia and Hypoxia in the Wistar Audiogenic Rat (WAR) Strain

Autor(es)

Granjeiro, Erica Maria ^{[1, 2]} ; da Silva, Glauber S. F. ^{[1, 3]} ; Giusti, Humberto ^[1] ; Oliveira, Jose Antonio ^[1] ; Glass, Mogens Lesner ^[1] ; Garcia-Cairasco, Norberto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Physiol, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Estadual Feira de Santana UEFS, Dept Ciencias Biol DCBIO, Av Transnordestina S-N, BR-44036900 Feira De Santana, BA - Brazil

^[3] Sao Paulo State Univ UNESP FCAV, Dept Morphol & Anim Physiol, Via Acesso Prof Paulo D Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 11, n. 5 MAY 5 2016.
• Citações Web of Science: 5

Resumo

Introduction We investigated the behavioral, respiratory, and thermoregulatory responses elicited by acute exposure to both hypercapnic and hypoxic environments in Wistar audiogenic rats (WARs). The WAR strain represents a genetic animal model of epilepsy. Methods Behavioral analyses were performed using neuroethological methods, and flowcharts were constructed to illustrate behavioral findings. The body plethysmography method was used to obtain pulmonary ventilation (VE) measurements, and body temperature (Tb) measurements were taken via temperature sensors implanted in the abdominal cavities of the animals. Results No significant difference was observed between the WAR and Wistar control group with respect to the thermoregulatory response elicited by exposure to both acute hypercapnia and acute hypoxia (p>0.05). However, we found that the VE of WARs was attenuated relative to that of Wistar control animals during exposure to both hypercapnic (WAR: 133 +/- 11% vs. Wistar: 243 +/- 23%, p<0.01) and hypoxic conditions (WAR: 138 +/- 8% vs. Wistar: 177 +/- 8%; p<0.01). In addition, we noted that this ventilatory attenuation was followed by alterations in the behavioral responses of these animals. Conclusions Our results indicate that WARs, a genetic model of epilepsy, have important alterations in their ability to compensate for changes in levels of various arterial blood gasses. WARs present an attenuated ventilatory response to an increased PaCO2 or decreased PaO2, coupled to behavioral changes, which make them a suitable model to further study respiratory risks associated to epilepsy. (AU)

• Processo FAPESP: 07/50261-4 - Neurociências, epilepsia e arte: caracterização interdisciplinar da cepa WAR e modelos de epilepsia correlacionados: as oficinas Da Vinci, divulgação científica e quebras de estigmas em epilepsia
• Beneficiário: Norberto Garcia Cairasco
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 06/60768-6 - Avaliacao da expressao do sistema ocitocinergico em ratos da cepa wistar audiogenic rat (war).
• Beneficiário: Simone Saldanha Marroni
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 05/56447-7 - Pesquisa através de imagens por ressonância magnética de alto campo destinadas a estudos em humanos
• Beneficiário: João Pereira Leite
• Modalidade de apoio: Auxílio à Pesquisa - Programa CINAPCE - Temático
• Processo FAPESP: 13/17606-9 - Serotonina e controle respiratório em vertebrados
• Beneficiário: Glauber dos Santos Ferreira da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores